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Mouse CXCR6 Antibody

Catalog #: MAB2145
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2145-100
MAB2145-SP
Detection of CXCR6 in BaF3 Mouse Cell Line Transfected with Mouse CXCR6 and eGFP by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR6 Antibody Summary

Species Reactivity
Mouse
Specificity
Stains mouse CXCR6-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2B Clone # 221002
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with mouse CXCR6
Met1-Leu351
Accession # Q9EQ16
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR6 antibody in BaF3 Mouse Cell Line Transfected with Mouse CXCR6 and eGFP antibody by Flow Cytometry. View Larger

Detection of CXCR6 in BaF3 Mouse Cell Line Transfected with Mouse CXCR6 and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP was stained with and either (A) Rat Anti-Mouse CXCR6 Monoclonal Antibody (Catalog # MAB2145) or (B) Rat IgG2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Entrez Gene IDs
10663 (Human); 80901 (Mouse); 100124593 (Rat)
Alternate Names
Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186

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Citations for Mouse CXCR6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The role of PKC and PKD in CXCL12 and CXCL13 directed malignant melanoma and acute monocytic leukemic cancer cell migration
    Authors: Hamshaw, I;Ellahouny, Y;Malusickis, A;Newman, L;Ortiz-Jacobs, D;Mueller, A;
    Cellular signalling
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Hepatectomy leads to loss of TRAIL-expressing liver NK cells via downregulation of the CXCL9-CXCR3 axis in mice
    Authors: T Yano, M Ohira, R Nakano, Y Tanaka, H Ohdan
    PLoS ONE, 2017-10-31;12(10):e0186997.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The chemokine receptor CXCR6 contributes to recruitment of bone marrow-derived fibroblast precursors in renal fibrosis.
    Authors: Xia Y, Yan J, Jin X, Entman M, Wang Y
    Kidney Int, 2014-03-19;86(2):327-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. The chemokine CXCL16 induces migration and invasion of glial precursor cells via its receptor CXCR6.
    Authors: Hattermann K, Ludwig A, Gieselmann V, Held-Feindt J, Mentlein R
    Mol. Cell. Neurosci., 2008-04-10;39(1):133-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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