Mouse Cystatin B Antibody

Catalog #: MAB14091 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB14091
MAB14091-SP

All Cystatin B Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cystatin B Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cystatin B in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Cystatin B is observed and no cross-reactivity with rhCystatin A, C, D, E/M, F, S, SA, rhFetuin A, rhFetuin B, rhHPRG, or rhKininogen is observed.

Source

Monoclonal Rat IgG_2A Clone # 227807

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Cystatin B
Met2-Phe98
Accession # Q62426

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of mouse spinal cord

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin B

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Cystatin B-deficient mice have increased expression of proteolysis, apoptosis and glial activation genes, which is consistent with the pathology found in the mouse model of human progressive myoclonus epilepsy (EPM1) (3). The mouse Cystatin B consists of 98 amino acid residues (4).

References

Abrahamson, M. (1994) Methods Enzymol. 244:685.
Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
Lieuallen, K. et al. (2001) Hum. Mol. Genet. 10:1867.
Pennacchio, L.A. and R.M. Myers (1996) Genome Res. 6:1103.

Entrez Gene IDs

1476 (Human); 13014 (Mouse)

Alternate Names

CPI-B; CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B; CSTB; cystatin B (stefin B); Cystatin B; EPM1; Liver thiol proteinase inhibitor; PME; Stefin B; stefin-B