Mouse Cystatin D/Cst10 Antibody

Catalog #: AF6255 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6255
AF6255-SP

Detection of Mouse Cystatin D/Cst10 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cystatin D/Cst10 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cystatin D/Cst10 in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human (rh) Cst1, rhCst2, and rhCst3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cystatin D/Cst10
Asn30-Phe148
Accession # NP_067380

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Cystatin D/Cst10 by Western Blot. Western blot shows lysates of mouse salivary gland tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Cystatin D/Cst10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6255) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cystatin D/Cst10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin D/Cst10

Cystatin D (Cystatin 10; also Carminerin) is a 17 kDa member of the type 2 cystatin family of proteins. It is expressed in salivary gland, skin, prehypertrophic and hypertrophic chondrocytes. Within the context of endochondral ossification, it may both induce chondrocyte apoptosis and initiate the process of cartilage calcification. Although Cystatin D contains a key cystatin motif (Gln-x-Val-x-Gly), Cystatin D does not appear to inhibit cysteine proteases. Mouse Cystatin D is 148 amino acids (aa) in length. It contains one cystatin-like domain (aa 39-145). There are two potential splice variants. One shows a deletion of aa 84-121, while a second shows a Val substitution for aa 122-148. Full-length mouse Cystatin D shares 78% aa identity with rat Cystatin D. There appears to be no direct human counterpart to mouse Cystatin D, as both human cystatin-C and -D share less that 40% aa identity with mouse Cystatin D.

Entrez Gene IDs

58214 (Mouse); 366219 (Rat)

Alternate Names

Cst10; Cystatin 10; cystatin D; Cystatin D/Cst10; DD72