Mouse DcTRAILR1/TNFRSF23 Antibody

Catalog #: MAB2378 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2378
MAB2378-SP

DcTRAIL R1/TNFRSF23 in Mouse Splenocytes.

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Mouse DcTRAILR1/TNFRSF23 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DcTRAIL R1/TNFRSF23 in direct ELISAs and Western blots. In Western blots, less than 10% cross-reactivity with recombinant mouse (rm) 4-1BB Ligand, recombinant human (rh) BAFF R, rhDcTRAIL R3, rmFas, rmOPG, and rhTRAIL R4 is observed and no cross‑reactivity with rmCD27, rmCD30, rmDR3, rhDR6, rmEDAR, recombinant feline Fas, rhFas, recombinant rat Fas, rmGITR, rmHVEM, rmLymphotoxin beta R, rmNGF R, rmOX40, rmTNF RI, rmTNF RII, rhTRAIL R1, or rhTROY is observed.

Source

Monoclonal Rat IgG_2A Clone # 270012

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DcTRAIL R1/TNFRSF23
Met30-Ser156
Accession # Q9ER63

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse DcTRAIL R1/TNFRSF23 under non-reducing conditions only

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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DcTRAIL R1/TNFRSF23 in Mouse Splenocytes. DcTRAIL R1/TNFRSF23 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse DcTRAIL R1/TNFRSF23 Mono-clonal Antibody (Catalog # MAB2378) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DcTRAILR1/TNFRSF23

DcTRAIL R1, also known as TNFRH1 and mSOB, is a GPI-anchored TNF receptor superfamily member that lacks a cytoplasmic death domain and does not induce apoptosis. No known human homolog of mouse DcTRAIL R1 is known.

Long Name

Decoy TRAIL Receptor 1

Entrez Gene IDs

79201 (Mouse)

Alternate Names

APO-3;DDR3;DR3;GEF720;LARD;PLEKHG5;TNFRSF12;TNFRSF25;TR3;TRAMP;Tumor necrosis factor receptor superfamily member 25;WSL-1;WSL-LR; DcTRAIL R1; DcTRAILR1; SOB; TNFRH1; TNFRSF23