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Mouse E-Selectin/CD62E Quantikine ELISA Kit

Catalog #: MES00
19 Citations Datasheet / COA / SDS
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MES00
Mouse E-Selectin/CD62E ELISA Standard Curve
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Citations (19)
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse E-Selectin/CD62E Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)
Sensitivity
4.7 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse E-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse soluble E-Selectin (sE-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sE-selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sE-selectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sE-selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sE-selectin.

Recovery

The recovery of mouse sE-Selectin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 95 83-102
EDTA Plasma (n=4) 101 78-120
Serum (n=10) 98 83-118

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with high concentrations of mouse sE-Selectin in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse E-Selectin/CD62E ELISA Linearity

Scientific Data

N/A Mouse E-Selectin/CD62E ELISA Standard Curve View Larger

Mouse E-Selectin/CD62E ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Selectin/CD62E

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

Entrez Gene IDs:
6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)
Alternate Names:
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse E-Selectin/CD62E Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Pre-Administration of PLX-R18 Cells Protects Mice from Radiation-Induced Hematopoietic Failure and Lethality
    Authors: VP Kumar, S Biswas, GP Holmes-Ham, M Sheleg, S Stone, B Legesse, R Ofir, SP Ghosh
    Genes, 2022-09-28;13(10):.
    Species: Mouse
    Sample Types: Serum
  2. Analysis of oxidative stress, inflammation and endothelial function following intravenous iron in chronic kidney disease in the Iron and Heart Trial
    Authors: X Kassianide, V Allgar, IC Macdougall, PA Kalra, S Bhandari
    Scientific Reports, 2022-04-27;12(1):6853.
    Species: Human
    Sample Types: Serum
  3. Quercetin Completely Ameliorates Hypoxia-Reoxygenation-Induced Pathophysiology Severity in NY1DD Transgenic Sickle Mice: Intrinsic Mild Steady State Pathophysiology of the Disease in NY1DD Is Also Reversed
    Authors: S Thangaswam, CA Branch, K Ambadipudi, SA Acharya
    Biomolecules, 2021-10-06;11(10):.
    Species: Mouse
    Sample Types: Plasma
  4. Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease
    Authors: J Venugopal, J Wang, J Mawri, C Guo, D Eitzman
    Haematologica, 2021-09-01;0(0):.
    Species: Mouse
    Sample Types: Plasma
  5. Hydroxychloroquine reverses the prothrombotic state in a mouse model of antiphospholipid syndrome: Role of reduced inflammation and endothelial dysfunction
    Authors: S Miranda, P Billoir, L Damian, PA Thiebaut, D Schapman, M Le Besnera, F Jouen, L Galas, H Levesque, V Le Cam-Duc, R Joannides, V Richard, Y Benhamou
    PLoS ONE, 2019-03-14;14(3):e0212614.
    Species: Mouse
    Sample Types: Plasma
  6. Simvastatin attenuates endothelial activation through 15-epi-lipoxin A4 production in murine chronic Chagas cardiomyopathy
    Authors: Fabiola González-H
    Antimicrob. Agents Chemother, 2017-02-23;0(0):.
    Species: Mouse
    Sample Types: Serum
  7. Bacterial lipoprotein TLR2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo.
    Authors: Shin HS, Xu F, Bagchi A, Herrup E, Wilhelmsen K
    J. Immunol., 2010-12-17;186(2):1119-30.
    Species: Mouse
    Sample Types: Plasma
  8. Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice.
    Authors: Deng Y, Edin ML, Theken KN, Schuck RN, Flake GP, Kannon MA, DeGraff LM, Lih FB, Foley J, Bradbury JA, Graves JP, Tomer KB, Falck JR, Zeldin DC, Lee CR
    FASEB J., 2010-11-08;25(2):703-13.
    Species: Mouse
    Sample Types: Cell Lysates
  9. Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene.
    Authors: Sio SW, Moochhala S, Lu J
    Am. J. Respir. Crit. Care Med., 2009-10-01;181(1):36-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Prevention of murine antiphospholipid syndrome by BAFF blockade.
    Authors: Kahn P, Ramanujam M, Bethunaickan R, Huang W, Tao H, Madaio MP, Factor SM, Davidson A
    Arthritis Rheum., 2008-09-01;58(9):2824-34.
    Species: Mouse
    Sample Types: Serum
  11. Aspergillus fumigatus stimulates leukocyte adhesion molecules and cytokine production by endothelial cells in vitro and during invasive pulmonary disease.
    Authors: Chiang LY, Sheppard DC, Gravelat FN, Patterson TF, Filler SG
    Infect. Immun., 2008-05-19;76(8):3429-38.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Vascular dysfunction in a murine model of severe hemolysis.
    Authors: Frei AC, Guo Y, Jones DW, Pritchard KA, Fagan KA, Hogg N, Wandersee NJ
    Blood, 2008-05-13;112(2):398-405.
    Species: Mouse
    Sample Types: Plasma
  13. Apolipoprotein C-I is crucially involved in lipopolysaccharide-induced atherosclerosis development in apolipoprotein E-knockout mice.
    Authors: Westerterp M, Berbee JF, Pires NM, van Mierlo GJ, Kleemann R, Romijn JA, Havekes LM, Rensen PC
    Circulation, 2007-10-22;116(19):2173-81.
    Species: Mouse
    Sample Types: Plasma
  14. Endogenous hydrogen sulfide regulates leukocyte trafficking in cecal ligation and puncture-induced sepsis.
    Authors: Zhang H, Zhi L, Moochhala SM, Moore PK, Bhatia M
    J. Leukoc. Biol., 2007-06-28;82(4):894-905.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Toll-like receptor 4 signal transduction inhibitor, M62812, suppresses endothelial cell and leukocyte activation and prevents lethal septic shock in mice.
    Authors: Nakamura M, Shimizu Y, Sato Y, Miyazaki Y, Satoh T, Mizuno M, Kato Y, Hosaka Y, Furusako S
    Eur. J. Pharmacol., 2007-06-05;569(3):237-43.
    Species: Mouse
    Sample Types: Plasma
  16. Cell-based angiopoietin-1 gene therapy for acute lung injury.
    Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
    Am. J. Respir. Crit. Care Med., 2007-02-22;175(10):1014-26.
    Species: Mouse
    Sample Types: Plasma
  17. A coagulation factor VII deficiency protects against acute inflammatory responses in mice.
    Authors: Xu H, Ploplis VA, Castellino FJ
    J. Pathol., 2006-12-01;210(4):488-96.
    Species: Mouse
    Sample Types: Plasma
  18. Delayed inflammatory responses to endotoxin in fibrinogen-deficient mice.
    Authors: Cruz-Topete D, Iwaki T, Ploplis VA, Castellino FJ
    J. Pathol., 2006-11-01;210(3):325-33.
    Species: Mouse
    Sample Types: Plasma
  19. Transgenic flavonoid tomato intake reduces C-reactive protein in human C-reactive protein transgenic mice more than wild-type tomato.
    Authors: Rein D, Schijlen E, Kooistra T, Herbers K, Verschuren L, Hall R, Sonnewald U, Bovy A, Kleemann R
    J. Nutr., 2006-09-01;136(9):2331-7.
    Species: Mouse
    Sample Types: Plasma

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