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Mouse EBI3 Antibody

Catalog #: MAB18341
8 Citations 1 Review Datasheet / COA / SDS
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MAB18341
MAB18341-SP
Detection of Mouse IL‑27/IL‑35 EBI3 Subunit by Western Blot.
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Citations (8)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EBI3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-27/IL-35 EBI3 Subunit in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human IL‑27/IL-35 EBI3 subunit.
Source
Monoclonal Rat IgG2A Clone # 355022
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑27 heterodimer
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IL-27/IL-35 EBI3 Subunit antibody by Western Blot. View Larger

Detection of Mouse IL‑27/IL‑35 EBI3 Subunit by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with mouse EBI3. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit Monoclonal Antibody (Catalog # MAB18341) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-27/IL-35 EBI3 Subunit at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBI3

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.

Long Name
Epstein-Barr virus induced gene 3
Entrez Gene IDs
10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey)
Alternate Names
cytokine receptor; EBI3; EBI-3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 beta; IL-27 subunit beta; IL27B; IL-27B; IL35 EBI3; IL-35 EBI3; interleukin-27 subunit beta

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Citations for Mouse EBI3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. B cell treatment promotes a neuroprotective microenvironment after traumatic brain injury through reciprocal immunomodulation with infiltrating peripheral myeloid cells
    Authors: Dwyer, LJ;Maheshwari, S;Levy, E;Poznansky, MC;Whalen, MJ;S�rbulescu, RF;
    Journal of neuroinflammation
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Interleukin-35 Prevents the Elevation of the M1/M2 Ratio of Macrophages in Experimental Type 1 Diabetes
    Authors: Z Luo, C Soläng, R Larsson, K Singh
    International Journal of Molecular Sciences, 2022-07-19;23(14):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. A novel cytokine consisting of the p40 and EBI3 subunits suppresses experimental autoimmune arthritis via reciprocal regulation of Th17 and Treg cells
    Authors: Seon-Yeong Lee, Su-Jin Moon, Young-Mee Moon, Hyeon-Beom Seo, Jun-Geol Ryu, A Ram Lee et al.
    Cellular & Molecular Immunology
  4. Macrophage-mediated RON signaling supports breast cancer growth and progression through modulation of IL-35
    Authors: SJ Ruiz-Torre, JR Bourn, NM Benight, BG Hunt, C Lester, SE Waltz
    Oncogene, 2021-11-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunodepletion
  5. IL-30� (IL-27A): a familiar stranger in immunity, inflammation, and cancer
    Authors: B Min, D Kim, MJ Feige
    Experimental & Molecular Medicine, 2021-05-28;53(5):823-834.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  6. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
    Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
    J Immunol, 2013-12-11;192(2):804-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. IL-35 stimulation of CD39+ regulatory T cells confers protection against collagen II-induced arthritis via the production of IL-10.
    Authors: Kochetkova I, Golden S, Holderness K
    J. Immunol., 2010-05-10;184(12):7144-53.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  8. Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10.
    Authors: Ilarregui JM, Croci DO, Bianco GA, Toscano MA, Salatino M, Vermeulen ME, Geffner JR, Rabinovich GA
    Nat. Immunol., 2009-08-09;10(9):981-91.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Mouse EBI3 Antibody

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Mouse IL-27/IL-35 EBI3 Subunit Antibody
By Anonymous on 08/06/2018
Application: WB Sample Tested: Cell Culture Supernates Species: Mouse