Mouse Endoglycan/PODXL2 Biotinylated Antibody

Catalog #: BAF3534 Datasheet / COA / SDS

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BAF3534

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Mouse Endoglycan/PODXL2 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Endoglycan/PODXL2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Endoglycan/PODXL2 isoform 1
Gly30-Gly498
Accession # Q8CAE9.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Endoglycan/PODXL2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglycan/PODXL2

Endoglycan, also named podocalyxin-like 2 protein, is a type I transmembrane glycoprotein that belongs to the CD34/podocalyxin family of sialomucins that are expressed on endothelial cells and hematopoietic precursors, vascular smooth muscle cells, and some leukocyte subpopulations. The extracellular domain of Endoglycan contains an N-terminal acidic domain with several polyglutamate tracts, a mucin-like domain and a membrane proximal globular domain. Unlike other sialomucins, Endoglycan contains chondroitin sulfate modifications. Within the extracellular domain, mouse endoglycan shares 73% and 96% aa sequence identity with human and rat endoglycan, respectively.

Entrez Gene IDs

50512 (Human); 319655 (Mouse)

Alternate Names

Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2