Mouse ENPP-7/Alk-SMase Biotinylated Antibody

Catalog #: BAF5160 Datasheet / COA / SDS

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BAF5160

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Mouse ENPP-7/Alk-SMase Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ENPP‑7/Alk‑SMase in Western blots. In Western blots, approximately 50% cross‑reactivity with recombinant human ENPP-7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ENPP‑7/Alk‑SMase
Ala22-Gln421
Accession # NP_001025462

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse ENPP-7/Alk-SMase (Catalog # 5160-EN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ENPP-7/Alk-SMase

ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is expressed in the intestines and in bile (1). It shares 30 - 36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies show a decrease in ENPP7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP7 in human colon cancer (4, 5).

References

Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
Wu, J. et al. (2006) Biochem J. 394:299.
Hertervig, E. et al. (1996) Cancer. 79:448.
Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 7

Entrez Gene IDs

339221 (Human); 238011 (Mouse); 303729 (Rat)

Alternate Names

Alkaline sphingomyelin phosphodiesterase; alkaline sphingomyelinase; Alk-SMase; EC 3.1.4.12; ectonucleotide pyrophosphatase/phosphodiesterase 7; ectonucleotide pyrophosphatase/phosphodiesterase family member 7; E-NPP 7; ENPP7; ENPP-7; Intestinal alkaline sphingomyelinase; MGC50179; NPP7; NPP-7