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Mouse Ephrin-A1 Biotinylated Antibody

Catalog #: BAF602
1 Citation Datasheet / COA / SDS
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BAF602
Product Details
Citations (1)
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Mouse Ephrin-A1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Ephrin-A1 in Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Ephrin-A5 is observed and less than 5% cross-reactivity with recombinant mouse (rm) Ephrin-A2, rmEphrin-B1, rmEphrin-B2, rhEphrin-B3, rhEphrin-A3, and rmEphrin-A4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A1
Asp19-Ser182
Accession # P52793
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Ephrin-A1 Fc Chimera (Catalog # 602-A1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A1

Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to interferon alpha.

Entrez Gene IDs
1942 (Human); 13636 (Mouse)
Alternate Names
B61; ECKLG; EFL1; EFL-1; EFNA1; EPH-related receptor tyrosine kinase ligand 1; EphrinA1; Ephrin-A1; EPLG1TNF alpha-induced protein 4; Immediate early response protein B61; LERK1; LERK1LERK-1; ligand of eph-related kinase 1; TNFAIP4B61; Tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4

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Citation for Mouse Ephrin-A1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ADAM12-cleaved ephrin-A1 contributes to lung metastasis.
    Authors: Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y
    Oncogene, 2013-05-20;33(17):2179-90.
    Species: Mouse
    Sample Types: Serum, Whole Tissue
    Applications: ELISA Development (Capture), IHC

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