Mouse FCRN Antibody Summary
Ser22-Ser301
Accession # AAH03786
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse FCRN by Western Blot. Western blot shows lysates of mouse liver tissue, mouse small intestine tissue, and mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FCRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for FCRN at approximately 48 to 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of FCRN in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse FCRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6775, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FCRN
FcRn (IgG receptor FcRn large subunit p51/ alpha -chain) is a 44-52 kDa member of the Ig superfamily. In mouse, it is expressed by intestinal epithelium of neonates and fetal yolk sac endoderm during pregnancy. Together with p14/ beta 2-Microglobulin, FcRn forms an MHC I-like noncovalent heterodimer termed the FcRn complex. In rodent, FcRn transports IgG in milk into the intestine, and IgG from the mother's blood into the mouse via endodermal FcRn linked to a special Fc gamma RIIb macrophage receptor. It is also expressed by select macrophages and DCs, and participates in endosomal-associated antigen presentation to CD4+ T cells. Mature mouse FcRn is a 344 amino acid (aa) type I transmembrane glycoprotein. It contains a 276 aa extracellular region (aa 22-297) that contains an Ig-like domain (aa 201-288). The FcRn complex forms a homodimer when binding IgG. One potential splice variant shows a four aa insertion after Gly109. Over aa 22-301 of the splice variant (GenBank #:AAH03786), mouse FcRn shares 91% and 68% aa identity with rat and human FcRn, respectively.
Product Datasheets
Citations for Mouse FCRN Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Megalin Orchestrates FcRn Endocytosis and Trafficking
Authors: E Dahlke, Y Anan, LM Klie, AE Hartkopf, F Theilig
Cells, 2022-12-22;12(1):.
Species: Mouse, Opossum
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension
Authors: ND Arnold, JA Pickworth, LE West, S Dawson, JA Carvalho, H Casbolt, AT Braithwait, J Iremonger, L Renshall, V Germaschew, M McCourt, P Bland-Ward, H Kowash, AG Hameed, AMK Rothman, MG Frid, AA Roger Thom, HR Evans, M Southwood, NW Morrell, DC Crossman, MKB Whyte, KR Stenmark, CM Newman, DG Kiely, SE Francis, A Lawrie
Nat Commun, 2019-11-15;10(1):5183.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization Control -
Abl family tyrosine kinases govern IgG extravasation in the skin in a murine pemphigus model
Authors: S Ono, G Egawa, T Nomura, A Kitoh, T Dainichi, A Otsuka, S Nakajima, M Amagai, F Matsumoto, M Yamamoto, Y Kubota, T Takai, T Honda, K Kabashima
Nat Commun, 2019-09-30;10(1):4432.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Neonatal Fc Receptor and Complement Fixation Facilitate Prophylactic Vaccine-Mediated Humoral Protection against Viral Infection in the Ocular Mucosa
Authors: DJ Royer, MM Carr, HR Gurung, WP Halford, DJJ Carr
J. Immunol., 2017-07-31;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Distribution of FcRn Across Species and Tissues
Authors: S Latvala, B Jacobsen, MB Otteneder, A Herrmann, S Kronenberg
J. Histochem. Cytochem., 2017-04-12;0(0):2215541770509.
Species: Human, Mouse, Primate, Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells
Nat Commun, 2016-07-18;7(0):12175.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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