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Mouse FGF-23 Antibody

Catalog #: MAB26291
22 Citations Datasheet / COA / SDS
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MAB26291
MAB26291-SP
FGF‑23 in Mouse Brain.
6 Images
Product Details
Citations (22)
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Mouse FGF-23 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FGF-23 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 50% cross-reactivity with recombinant human (rh) FGF-23. In Western blots, this antibody does not cross-react with rhFGF-3, -4, -5, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, acidic, basic, rmFGF-8b, -8c, -15, or -21.
Source
Monoclonal Rat IgG2A Clone # 283507
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF-23
Tyr25-Val251 (Arg179Gln)
Accession # Q9EPC2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FGF-23 antibody in Mouse Brain by Immunohistochemistry (IHC-P) by Immunohistochemistry (IHC-Fr). View Larger

FGF‑23 in Mouse Brain. FGF-23 was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse FGF-23 Monoclonal Antibody (Catalog # MAB26291) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Detection of Rat FGF-23 by Immunohistochemistry View Larger

Detection of Rat FGF-23 by Immunohistochemistry Immunofluorescence in the kidney of hemi-nephrectomized rats fed a high-P diet.FGF23 immunofluorescence (green). alpha SMA immunofluorescence (red). Merge: FGF23 (green), alpha SMA (red), and DAPI (blue). ×400: high magnification. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29518087), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Rat FGF-23 by Western Blot View Larger

Detection of Rat FGF-23 by Western Blot FGF23 expression in the partial nephrectomy rat model.(a) Serum FGF23 concentration. (b) FGF23 mRNA expression in the kidney. Sham group was used as a normalization control. (c) Western blot of FGF23 in the kidney. (d) Histology in the kidney. HE: hematoxylin-eosin staining. MT: Masson’s trichrome staining to evaluate fibrosis. VK: Von Kossa staining to evaluate calcification. *P<0.05, **P<0.01, ***P<0.001; sham group (n = 6), partial nephrectomy mild group (PN mild) (n = 6), partial nephrectomy severe group (PN severe) (n = 6). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29518087), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Rat FGF-23 by Western Blot View Larger

Detection of Rat FGF-23 by Western Blot FGF23 expression in hemi-nephrectomized rats fed a high-P diet.(a) Serum FGF23 concentration. (b) FGF23 mRNA expression in the bone. NP sham group was used as a normalization control. (c) FGF23 mRNA expression in the kidney. NP sham group was used as a normalization control. (d) Western blot of FGF23 in the kidney. GAPDH was used as an internal control. Each value shown represents the mean ± SEM; *P<0.05, **P<0.01; NP sham group (n = 8), NP Nx group (n = 7), HP sham group (n = 7) and HP Nx group (n = 9). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29518087), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Rat FGF-23 by Immunohistochemistry View Larger

Detection of Rat FGF-23 by Immunohistochemistry Immunohistochemistry and in situ hybridization in the kidney of partial nephrectomy rat model.FGF23: FGF23 immunohistochemistry (brown). Osteopontin: osteopontin immunohistochemistry (brown). ×200: high magnification. FGF23 in situ hybridization (red spots; arrow: positive cells). ×400: high magnification. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29518087), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Rat FGF-23 by Immunohistochemistry View Larger

Detection of Rat FGF-23 by Immunohistochemistry Immunohistochemistry and in situ hybridization in the kidney of hemi-nephrectomized rats fed a high-P diet.FGF23: FGF23 immunohistochemistry (brown). Osteopontin: osteopontin immunohistochemistry (brown). ×200: high magnification. FGF23 in situ hybridization (red spots. arrow: positive cells). ×400: high magnification. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29518087), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-23

Fibroblast growth factor 23 (FGF-23) is a 30-32 kDa member of the FGF gene family. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members exist as highly diffusible molecules that is attributed to poor ECM/heparin sulfate binding (3-6). The cDNA for mouse FGF-23 predicts a 251 aa polypeptide that contains a 24 aa signal sequence and a 227 aa mature region (7). Mature mouse FGF-23 shows 72% aa identity to human FGF-23 (8). The FGF-19 subfamily shares an unusual receptor configuration. The standard model for FGF signaling requires an FGF:FGF R:heparin sulfate complex. Given FGF-23’s minimal association with heparin, a substitute termed ( alpha -) Klotho has evolved that serves the same function. Although FGF-23 binds to the widely expressed “c” isoforms of FGF R1 and 3 plus FGF R4, Klotho has a restricted distribution that limits FGF-23 activity (10-12). It should be noted that heparin-dependency has been reported for FGF-19 signaling, and this observation may extend to FGF-23 (13). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism and all three are induced by a nuclear receptor heterodimer that includes the retinoid X receptor (14-16). FGF-23 is considered a phosphatonin; that is, a molecule that reduces circulating plasma phosphate. It is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone that induces hypercalcemia, and circulatory volume loading. Upon binding to FGF-23 receptors on renal proximal tubular epithelium, two basic changes are seen. First, the enzyme responsible for generating the active form of vitamin D is suppressed, resulting in decreased levels of bioactive vitamin D. Since vitamin D promotes intestinal phosphate absorption, plasma phosphate declines. Second, the transporters responsible for phosphate resorption on renal epithelium are down regulated, resulting in decreased uptake from urine and again a decline in blood phosphorus (17, 18).

References
  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563. 
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
  4. Huang, X. et al. (2006) Mol. Carcinog. 45:934. 
  5. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
  6. Harmer, N.J. et al. (2004) Biochemistry 43:629.
  7. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
  8. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
  9. Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
  10. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  11. Urakawa, I. et al. (2006) Nature 444:770.
  12. Hurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
  13. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
  14. Moore, D.D. (2007) Science 316:1436.
  15. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
  16. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  17. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
  18. Liu, S. et al. (2007) Curr. Opin. Nephrol. Hypertens. 16:329.
Long Name
Fibroblast Growth Factor 23
Entrez Gene IDs
8074 (Human); 64654 (Mouse)
Alternate Names
ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor

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Citations for Mouse FGF-23 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. SIRT6-PAI-1 axis is a promising therapeutic target in aging-related bone metabolic disruption
    Authors: Aobulikasimu, A;Liu, T;Piao, J;Sato, S;Ochi, H;Okawa, A;Tsuji, K;Asou, Y;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Evocalcet rescues secondary hyperparathyroidism-driven cortical porosity in chronic kidney disease male rats
    Authors: Tomoka Hasegawa, Shin Tokunaga, Tomomaya Yamamoto, Mariko Sakai, Hiromi Hongo, Takehisa Kawata et al.
    Endocrinology
  3. FGF receptor inhibitor BGJ398 partially rescues osteoarthritis-like phenotype in older high molecular weight FGF2 transgenic mice via multiple mechanisms
    Authors: MM Hurley, JD Coffin, T Doetschman, C Valera, K Clarke, L Xiao
    Scientific Reports, 2022-09-24;12(1):15968.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Fibroblast Growth Factor 2 High Molecular Weight Isoforms in Dentoalveolar Mineralization
    Authors: Grethel Millington, Johnny Joseph, Liping Xiao, Anushree Vijaykumar, Mina Mina, Marja M. Hurley
    Calcified Tissue International
  5. Induction of somatopause in adult mice compromises bone morphology and exacerbates bone loss during aging
    Authors: M Dixit, S Duran-Orti, G Yildirim, SB Poudel, LD Louis, A Bartke, MB Schaffler, JJ Kopchick, S Yakar
    Aging Cell, 2021-11-23;0(0):e13505.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Localization of fibroblast growth factor 23 protein in the rat hypothalamus
    Authors: Stan R. Ursem, Charlene Diepenbroek, Vesna Bacic, Unga A. Unmehopa, Leslie Eggels, Clarissa M. Maya‐Monteiro et al.
    European Journal of Neuroscience
  7. Lack of PTEN in osteocytes increases circulating phosphate concentrations by decreasing intact fibroblast growth factor 23 levels
    Authors: M Kawai, S Kinoshita, K Ozono, T Michigami
    Scientific Reports, 2020-12-09;10(1):21501.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  8. Ablation of low‐molecular‐weight FGF2 isoform accelerates murine osteoarthritis while loss of high‐molecular‐weight FGF2 isoforms offers protection
    Authors: Patience Meo Burt, Liping Xiao, Thomas Doetschman, Marja M. Hurley
    Journal of Cellular Physiology
  9. FGF-23 Deficiency Impairs Hippocampal-Dependent Cognitive Function
    Authors: Ann M. Laszczyk, Dailey Nettles, Tate A. Pollock, Stephanie Fox, Melissa L. Garcia, Jing Wang et al.
    eNeuro
  10. Fibroblast growth factor 23 is upregulated in the kidney in a chronic kidney disease rat model
    Authors: H Sugiura, A Matsushita, M Futaya, A Teraoka, KI Akiyama, N Usui, N Nagano, K Nitta, K Tsuchiya
    PLoS ONE, 2018-03-08;13(3):e0191706.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Calcium and vitamin-D deficiency marginally impairs fracture healing but aggravates posttraumatic bone loss in osteoporotic mice
    Authors: Verena Fischer, Melanie Haffner-Luntzer, Katja Prystaz, Annika vom Scheidt, Björn Busse, Thorsten Schinke et al.
    Scientific Reports
  12. Targeted disruption of NF1 in osteocyte increases FGF23 and osteoid with osteomalacia-like bone phenotype
    Authors: N Kamiya, R Yamaguchi, O Aruwajoye, A Kim, G Kuroyanagi, M Phipps, NS Adapala, JQ Feng, HKW Kim
    J. Bone Miner. Res., 2017-05-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone
    Authors: A Sakurai, T Hasegawa, A Kudo, Z Shen, T Nagai, M Abe, T Yoshida, H Hongo, T Yamamoto, T Yamamoto, K Oda, PHL Freitas, M Li, H Sano, N Amizuka
    Biomed. Res., 2017-01-01;38(4):257-267.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Dietary phosphate supplement does not rescue skeletal phenotype in a mouse model for craniometaphyseal dysplasia
    Authors: I-Ping Chen
    J Negat Results Biomed, 2016-10-26;15(1):18.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Osteopontin protects against high phosphate-induced nephrocalcinosis and vascular calcification
    Authors: Neil J. Paloian, Elizabeth M. Leaf, Cecilia M. Giachelli
    Kidney International
  16. Posttranslational processing of FGF23 in osteocytes during the osteoblast to osteocyte transition
    Authors: Hiroyuki Yamamoto, Bruno Ramos-Molina, Adam N. Lick, Matthew Prideaux, Valeria Albornoz, Lynda Bonewald et al.
    Bone
  17. CYP24 inhibition as a therapeutic target in FGF23-mediated renal phosphate wasting disorders
    Authors: Xiuying Bai, Dengshun Miao, Sophia Xiao, Dinghong Qiu, René St-Arnaud, Martin Petkovich et al.
    Journal of Clinical Investigation
  18. Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs
    Authors: Z Liu, T Yamamoto, T Hasegawa, H Hongo, K Tsuboi, E Tsuchiya, M Haraguchi, M Abe, PH Freitas, A Kudo, K Oda, M Li, N Amizuka
    Biomed Res, 2016-01-01;37(2):141-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
    Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
    Kidney Int, 2014-01-08;85(6):1340-50.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Early chronic kidney disease–mineral bone disorder stimulates vascular calcification
    Authors: Yifu Fang, Charles Ginsberg, Toshifumi Sugatani, Marie-Claude Monier-Faugere, Hartmut Malluche, Keith A. Hruska
    Kidney International
  21. Renal phosphate wasting due to tumor-induced osteomalacia: a frequently delayed diagnosis.
    Authors: Gore MO, Welch BJ, Geng W, Kabbani W, Maalouf NM, Zerwekh JE, Moe OW, Sakhaee K
    Kidney Int., 2008-07-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. CKD-induced wingless/integration1 inhibitors and phosphorus cause the CKD-mineral and bone disorder.
    Authors: Fang Y, Ginsberg C, Seifert M, Agapova O, Sugatani T, Register T, Freedman B, Monier-Faugere M, Malluche H, Hruska K
    J Am Soc Nephrol, 2014-02-27;25(8):1760-73.

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