Mouse Frizzled-3 Biotinylated Antibody

Catalog #: BAM1001 Datasheet / COA / SDS

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BAM1001

All Frizzled-3 Antibodies

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Mouse Frizzled-3 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Frizzled-3 in direct ELISAs and Western blots. In immunohistochemistry detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and on human HEK 293 cells. In direct ELISAs, no cross‑reactivity with Frizzled-1, -4, -7 or -8 is observed.

Source

Monoclonal Rat IgG_2A Clone # 169310

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
Met1-Val157
Accession # Q61086

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Frizzled‑3

Immunohistochemistry

8-25 µg/mL

Immersion fixed frozen sections of mouse embryo (E13-15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-3

Frizzled-3 is a member of the Frizzled family of G-protein coupled receptors that have seven transmembrane domains and an extracellular CRD. Frizzled proteins function as receptors for Wnt proteins.

Entrez Gene IDs

7976 (Human); 14365 (Mouse)

Alternate Names

frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3