Mouse Galectin-2 Antibody

Catalog #: MAB6667 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6667
MAB6667-SP

Detection of Mouse Galectin‑2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Galectin-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Galectin-2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Galectin-2 is observed and no cross-reactivity with rhGalectin-4, -8, -10, recombinant mouse Galectin-1, -3, -7, or -9 is observed.

Source

Monoclonal Rat IgG_2A Clone # 747307

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Galectin-2
Ser2-Glu130
Accession # Q9CQW5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Galectin‑2 by Western Blot. Western blot shows lysates of mouse stomach tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Mouse Galectin-2 Monoclonal Antibody (Catalog # MAB6667) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin-2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Galectin‑2 in Mouse Stomach. Galectin-2 was detected in perfusion fixed frozen sections of mouse stomach using Rat Anti-Mouse Galectin-2 Monoclonal Antibody (Catalog # MAB6667) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to gastric pits. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-2

Galectin-2 (Gal-2; also LEG2, HL14 and L-14-II) is a 14-15 kDa member of the S-type (1-CRD type) lectin family of proteins. It is a potentially secreted, noncovalent homodimer that is expressed by stomach mucosal columnar epithelium, small intestine Goblet cells and keratinocytes. Galectin-2 is reported to bind to CD29, and initiate CD8⁺ T cell apoptosis. Structurally, it serves to bind nonsialylated poly-NAcetyllactosamine motifs. Mouse Galectin-2 is 130 amino acids (aa) in length. There is one galectin domain (aa 4-130) that contains one dimerization interface (aa 123-128). Full-length mouse Galectin-2 shares 86% and 66% aa identity with rat and human Galectin-2, respectively.

Entrez Gene IDs

3957 (Human); 107753 (Mouse); 171134 (Rat)

Alternate Names

Beta-galactoside-binding lectin L-14-II; GAL2; Gal-2; galectin 2; Galectin2; Galectin-2; HL14gal-2; Lactose-binding lectin 2; lectin, galactoside-binding, soluble, 2; LGALS2; MGC75071; S-Lac lectin 2