Mouse Galectin-7 Biotinylated Antibody

Catalog #: BAF1304 Datasheet / COA / SDS

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BAF1304

All Galectin-7 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Galectin-7 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Galectin-7 in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross‑reactivity with recombinant human (rh) Galectin‑7 is observed and less than 0.5% cross-reactivity with rhGalectin-1, recombinant mouse Galectin-3, rhGalectin-4, and rhGalectin‑8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Galectin‑7
Ser2-Phe136
Accession # Q99ML7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Galectin‑7 (Catalog # 1304-GA)

Mouse Galectin-7 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse Galectin‑7 Antibody (Catalog # MAB1304)

Standard: Recombinant Mouse Galectin-7 Protein (Catalog # 1304-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-7

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Mouse Galectin-7 is a prototype monomeric galectin. It is expressed in stratified epithelia and is significantly down-regulated in squamous cell carcinomas. Galectin-7 is a pro-apoptotic protein that is highly induced by the tumor suppressor protein p53. It functions intracellularly upstream of JNK activation to enhance cytochrome c release during apoptosis (5). Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re‑epithelialization of wounds (6).

References

Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
Hughes, R.C. (2002) Biochimie 83:667.
R&D Systems' Cytokine Bulletin, Summer (2002).
Kuwabara, I. et al. (2002) J. Biol. Chem. 277:3487.
Cao, Z. et al. (2002) J. Biol. Chem. 277:42299.

Entrez Gene IDs

3963 (Human); 16858 (Mouse)

Alternate Names

GAL7; Gal-7; galectin 7; Galectin7; Galectin-7; HKL-14; lectin, galactoside-binding, soluble, 7; LGALS7; LGALS7A; p53-induced gene 1 protein; PI7; PIG1; TP53I1