Mouse GDF-5/BMP-14 Antibody

Catalog #: MAB8531 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8531
MAB8531-SP

Alkaline Phosphatase Production Induced by GDF‑5 and Neutralization by Mouse GDF‑5 Antibody.

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Mouse GDF-5/BMP-14 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GDF-5/BMP-14 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) GDF-1, rmPro-GDF‑1, rmGDF-3, rmPro-GDF-3, rmGDF-6, rmGDF-7, rmGDF-8, rmGDF-9, rmPro-GDF-9, rmGDF-15, or recombinant human GDF-11 is observed.

Source

Monoclonal Rat IgG_2B Clone # 143004

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse GDF-5/BMP-14
Ala376-Arg495
Accession # P43027

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize GDF‑5-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 3 µg/mL Recombinant Mouse GDF‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Alkaline Phosphatase Production Induced by GDF‑5 and Neutralization by Mouse GDF‑5 Antibody. Recombinant Mouse GDF-5 (Catalog # 853-G5) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse GDF-5 (3 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GDF-5 Monoclonal Antibody (Catalog # MAB8531). The ND₅₀ is typically 2-10 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-5/BMP-14

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1) and BMP-14, is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF‑ beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N‑terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a subgroup within the BMP family. Like other TGF‑ beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

References

Storm, E.E. et al. (1994) Nature 368:639.
Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
Francis-West, P.H. et al. (1999) Development 126:1035.
Massague, J. et al. (2000) Genes and Dev. 14:627.
Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
Inada, M. et al. (1996) BBRC 222:317.

Long Name

Growth Differentiation Factor 5

Entrez Gene IDs

8200 (Human); 14563 (Mouse)

Alternate Names

BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2