Mouse GDF-9 Antibody

Catalog #: MAB739 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB739
MAB739-SP

Growth Inhibition Induced by GDF‑9 and Neutralization by Mouse GDF‑9 Antibody.

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Mouse GDF-9 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GDF-9 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 785123

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse GDF-9
Met1-Arg441
Accession # Q07105

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize GDF‑9-induced growth inhibition in the Mv1Lu mink lung epithelial cell line. The Neutralization Dose (ND₅₀) is typically 0.4-2.4 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse GDF‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Growth Inhibition Induced by GDF‑9 and Neutralization by Mouse GDF‑9 Antibody. Recombinant Mouse GDF-9 (739-G9) induces growth inhibition in the Mv1Lu mink lung epithelial cell line in the presence of in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Growth Inhibition elicited by Recombinant Mouse GDF-9 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GDF-9 Monoclonal Antibody (Catalog # MAB739). The ND₅₀ is typically 0.4-2.4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-9

Growth differentiation factor-9 (GDF-9) is a member of the transforming growth factor-beta (TGF-beta ) superfamily, and is an oocyte secreted paracrine factor essential for mammalian ovarian folliculogenesis (1‑2). Mouse GDF-9 is synthesized as a 441 amino acid (aa) prepropeptide that contains a 29 aa signal sequence, a 277 aa propeptide, and a 135 aa mature chain. Residues 340‑441 constitute a TGF-beta like domain. In addition, there is one potential site of N-linked glycosylation in the mature chain. Unlike other members of the TGF-beta superfamily, GDF-9 lacks the conserved cysteine residue that is believed to form the sole disulfide linkage between subunits in other family members (3). Mature mouse GDF-9 shares 90% aa sequence identity with mature human GDF 9. The protein is expressed throughout the development of the maturing follicle (2). GDF-9 functions as a paracrine factor in the regulation of granulosa cell proliferation and differentiation, and is essential for fertility (2, 4). Studies on GDF-9 null mice have demonstrated arrested follicular development at the primary follicle stage (5). Mouse GDF-9 induces Smad2 phosphorylation and inhibin production in rat diethylstilbestrol treated granulosa cells (6) and in human granulosa-luteal cells (7). The downstream signaling actions of GDF 9 are mediated by the type I receptor, activin receptor-like kinase 5 (ALK5), initiating the subsequent activation of Smad2 and Smad3 (2, 8). GDF 9 uses the BMP type II receptor (BMPRII) as its other signaling receptor (2, 9).

References