Mouse Glut2 Biotinylated Antibody

Catalog #: BAM1440
1 Citation Datasheet / COA / SDS

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BAM1440

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Mouse Glut2 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Glut2. Stains mouse Glut2-transfected NS0 cells but not NS0 control transfectants. It also detects Glut2 on mouse insulinoma beta TC-6 cells (2).

Source

Monoclonal Rat IgG_2B Clone # 205115

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

CHO Chinese hamster ovary cell line transfected with mouse Glut2
Met1-Val523
Accession # P14246

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

beta TC‑6 mouse beta cell insulinoma cell line

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Glut2 in beta TC‑6 Mouse Cell Line. Glut2 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Biotinylated Monoclonal Antibody (Catalog # BAM1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut2

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta -cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta -cells. Glut2 facilitates either glucose uptake or efflux from cells depending on the nutritional state (1).

References

Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
Poitout, V. et al. (1995) Diabetes 44:306.

Long Name

Glucose Transporter Type 2

Entrez Gene IDs

6514 (Human); 20526 (Mouse)

Alternate Names

GLUT2 Glucose transporter type 2, liver; Glut2; GLUT-2; SLC2A2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2, facilitated glucose transporter member 2

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Mouse Glut2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
Authors: Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O
J. Immunol., 2011-08-17;187(6):3096-103.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry