Home / Ly-6G/Ly-6C (Gr-1) / Mouse Gr-1/Ly-6G APC-conjugated Antibody

Mouse Gr-1/Ly-6G APC-conjugated Antibody

Catalog #: FAB1037A
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1037A-100
FAB1037A-025
Detection of Gr‑1/Ly‑6G in B220-Mouse Splenocytes by Flow Cytometry.
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Citations (5)
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Mouse Gr-1/Ly-6G APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.
Source
Monoclonal Rat IgG2B Clone # RB6-8C5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Gr-1/Ly-6G antibody in B220<SUP>-</SUP>Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Gr‑1/Ly‑6G in B220-Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References
  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse)
Alternate Names
Gr1; Gr-1; Ly-6C; Ly6G; Ly-6G; ly-6G.1; Ly-6G/Ly-6C (Gr-1); lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G

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Citations for Mouse Gr-1/Ly-6G APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Deletion of the Chemokine Binding Protein Gene from the Parapoxvirus Orf Virus Reduces Virulence and Pathogenesis in Sheep
    Authors: SB Fleming, C McCaughan, Z Lateef, A Dunn, LM Wise, NC Real, AA Mercer
    Front Microbiol, 2017-01-24;8(0):46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process
    Authors: Pedro Luiz Rosalen
    Phytomedicine, 2016-09-28;23(13):1583-1590.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
    Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
    Mucosal Immunol, 2014-01-15;7(5):1079-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
    Authors: Salvucci O, Jiang K, Gasperini P
    Haematologica, 2012-01-22;97(6):818-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Differential IL-23 requirement for IL-22 and IL-17A production during innate immunity against Salmonella enterica serovar Enteritidis.
    Authors: Siegemund S, Schutze N, Schulz S, Wolk K, Nasilowska K, Straubinger RK, Sabat R, Alber G
    Int. Immunol., 2009-03-18;21(5):555-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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