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Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody

Catalog #: FAB1037F
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1037F-100
FAB1037F-025
Detection of Gr‑1/Ly‑6G in Mouse Splenocytes by Flow Cytometry.
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Citations (5)
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Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.
Source
Monoclonal Rat IgG2B Clone # RB6-8C5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Gr-1/Ly-6G antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Gr‑1/Ly‑6G in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F) or (B) Rat IgG2BFluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References
  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse)
Alternate Names
Gr1; Gr-1; Ly-6C; Ly6G; Ly-6G; ly-6G.1; Ly-6G/Ly-6C (Gr-1); lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G

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Citations for Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Integrating network pharmacology and transcriptomic validation to investigate the efficacy and mechanism of Mufangji decoction preventing lung cancer
    Authors: F Gao, Y Niu, L Sun, W Li, H Xia, Y Zhang, S Geng, Z Guo, H Lin, G Du
    Journal of ethnopharmacology, 2022-07-30;0(0):115573.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  2. MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
    Authors: Salvucci O, Jiang K, Gasperini P
    Haematologica, 2012-01-22;97(6):818-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-17RA signaling amplifies antibody-induced arthritis.
    Authors: Sadik CD, Kim ND, Alekseeva E, Luster AD
    PLoS ONE, 2011-10-20;6(10):e26342.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Chemokine homeostasis vs. chemokine presentation during severe acute lung injury: the other side of the Duffy antigen receptor for chemokines.
    Authors: Zarbock A, Bishop J, Muller H, Schmolke M, Buschmann K, Van Aken H, Singbartl K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010-01-08;298(3):L462-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. TREM-1 promotes survival during septic shock in mice.
    Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
    Eur. J. Immunol., 2007-02-01;37(2):456-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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