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Mouse HAI-1 Antibody

Catalog #: AF1141
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1141
AF1141-SP
Product Details
Citations (9)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse HAI-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HAI-1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human HAI‑1 is observed and less than 1% cross-reactivity with recombinant mouse HAI-2B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HAI-1
Glu30-Glu443 (Cys325Ser)
Accession # NP_058603
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse HAI‑1 (Catalog # 1141-PI)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse intestine
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse HAI‑1 (Catalog # 1141-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-1

Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

References
  1. Denda, et al. (2002) J. Biol. Chem. 277:14053.
  2. Shimomura, et al. (1997) J. Biol. Chem. 272:6370.
  3. Kataoka, et al. (2000) J. Biol. Chem. 275:40453.
Long Name
HGF Activator Inhibitor Type 1
Entrez Gene IDs
6692 (Human); 20732 (Mouse)
Alternate Names
HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1

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Citations for Mouse HAI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Proteolytic Cleavage of Podocin by Matriptase Exacerbates Podocyte Injury
    Authors: S Ozawa, M Matsubayas, H Nanaura, M Yanagita, K Mori, K Asanuma, N Kajiwara, K Hayashi, H Ohashi, M Kasahara, H Yokoi, H Kataoka, E Mori, T Nakagawa
    J. Biol. Chem., 2020-09-09;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  2. Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins
    Authors: CJ Wu, M Lu, X Feng, G Nakato, MC Udey
    Cells, 2020-04-21;9(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. PDZ-RhoGEF and LARG are essential for embryonic development and provide a link between thrombin and LPA receptors and Rho activation.
    Authors: Mikelis C, Palmby T, Simaan M, Li W, Szabo R, Lyons R, Martin D, Yagi H, Fukuhara S, Chikumi H, Galisteo R, Mukouyama Y, Bugge T, Gutkind J
    J Biol Chem, 2013-03-06;288(17):12232-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis.
    Authors: Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H
    Cancer Res, 2013-02-27;73(8):2659-70.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  5. Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation.
    Authors: Szabo, Roman, Uzzun Sales, Katiuchi, Kosa, Peter, Shylo, Natalia, Godiksen, Sine, Hansen, Karina K, Friis, Stine, Gutkind, J Silvio, Vogel, Lotte K, Hummler, Edith, Camerer, Eric, Bugge, Thomas H
    PLoS Genet, 2012-08-30;8(8):e1002937.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Immunoprecipitation
  6. Imbalance in the pro-hepatocyte growth factor activation system in bleomycin-induced lung fibrosis in mice.
    Authors: Phin S, Marchand-Adam S, Fabre A, Marchal-Somme J, Bantsimba-Malanda C, Kataoka H, Soler P, Crestani B
    Am. J. Respir. Cell Mol. Biol., 2009-05-15;42(3):286-93.
    Species: Mouse
    Sample Types: BALF, Whole Tissue
    Applications: IHC-P, Neutralization
  7. Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity.
    Authors: Szabo R, Hobson JP, List K, Molinolo A, Lin CY, Bugge TH
    J. Biol. Chem., 2008-08-19;283(43):29495-504.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Hepatocyte growth factor activator inhibitor-1 has a complex subcellular itinerary.
    Authors: Godiksen S, Selzer-Plon J, Pedersen ED, Abell K, Rasmussen HB, Szabo R, Bugge TH, Vogel LK
    Biochem. J., 2008-07-15;413(2):251-9.
    Species: Canine
    Sample Types: Whole Cells
    Applications: ICC
  9. Matriptase inhibition by hepatocyte growth factor activator inhibitor-1 is essential for placental development.
    Authors: Szabo R, Molinolo A, List K, Bugge TH
    Oncogene, 2006-09-18;26(11):1546-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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