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Mouse HAI-2 Ectodomain Antibody

Catalog #: AF1107
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1107
AF1107-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse HAI-2 Ectodomain Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HAI-2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human HAI-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HAI-2B
Arg30-Lys140
Accession # NP_001076017
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse HAI‑2 Ectodomain
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse liver

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2

Two alternatively spliced forms of HAI-2 are found in human tissues. HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain. Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trypsin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa.

Three alternatively spliced forms of HAI-2 have been found in mouse tissues. HAI-2A is a minor form expressed in mouse tissues. HAI-2B, with residues 37 through 93 spliced out and thus lack of the first Kunitz domain, is a major form in mouse tissues. HAI-2B is an effective inhibitor of both HGF activator and trypsin.

Long Name
HGF Activator Inhibitor Type 2
Entrez Gene IDs
10653 (Human); 20733 (Mouse)
Alternate Names
HAI2; HAI-2; HAI-2DIAR3; HAI2FLJ45571; Hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2

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Citations for Mouse HAI-2 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins
    Authors: CJ Wu, M Lu, X Feng, G Nakato, MC Udey
    Cells, 2020-04-21;9(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumors.
    Authors: Sales K, Friis S, Abusleme L, Moutsopoulos N, Bugge T
    Oncogene, 2014-12-08;34(35):4664-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity.
    Authors: Szabo R, Hobson JP, List K, Molinolo A, Lin CY, Bugge TH
    J. Biol. Chem., 2008-08-19;283(43):29495-504.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot

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