Mouse HSP10/EPF Antibody

Catalog #: AF2584 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2584
AF2584-SP

Detection of Mouse HSP10/EPF by Western Blot.

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All HSP10/EPF Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse HSP10/EPF Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse HSP10/EPF in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse HSP10/EPF
Ala2-Asp102
Accession # Q64433

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse HSP10/EPF by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP10/EPF at approximately 10 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Simple Western

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Detection of Mouse HSP10/EPF by Simple Western^TM. Simple Western lane view shows lysates of DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP10/EPF at approximately 9 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP10/EPF

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Protein 10 (HSP10), also known as chaperonin 10 (Cpn10), HSPE1, and Early Pregnancy Factor (EPF), is a 10 kDa heat shock protein that functions within the cell as a molecular chaperone and is associated with HSP60 in the mitochondria. Early pregnancy factor (EPF) has been identified as an extracellular homologue of HSP10.

Long Name

Heat Shock 10 kDa Protein 1

Entrez Gene IDs

3336 (Human); 69253 (Mouse)

Alternate Names

10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; Chaperonin 10 Homolog; Chaperonin 10; CPN10HSP10; Early-pregnancy factor; EPF; GroES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1 (chaperonin 10); HSP10; HSPE1