Mouse IFN-alpha 2/IFNA2 Antibody

Catalog #: MAB10149 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10149-100
MAB10149-SP

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-alpha 2/IFNA2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFN-alpha 2/IFNA2 in direct ELISA. In direct ELISAs, no cross-reactivity with mouse IFN-alpha 1, 4, 9, 11, 12 and 15 was observed.

Source

Monoclonal Rat IgG_2A Clone # 996586

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2
Cys24-Glu190
Accession # P01573

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with mouse anti-IFNA-2 Monoclonal Antibody (Catalog # MAB101491). This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 2/IFNA2

Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).

References

Pestka, S. (2007) J Biol Chem. 282:20047.
Paul, F. et al. (2015) Gene. 567(2):132.
Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
Pesch, V. et al. (2004). Journal of Virology, 78:8219.
Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.
Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.

Long Name

Interferon alpha 2

Entrez Gene IDs

3440 (Human); 15965 (Mouse); 298213 (Rat)

Alternate Names

alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765