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Mouse IFN-alpha / beta  R1 Antibody

Catalog #: AF3039
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3039
AF3039-SP
Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R1 Antibody.
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Citations (5)
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Mouse IFN-alpha / beta  R1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) IFN‑ alpha / beta  R2, rmIFN-gamma R1, and rmIFN-gamma R2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R1
Glu27-Thr429
Accession # P33896
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IFN-alpha / beta  R1 (Catalog # 3039-AB)
Neutralization
Measured by its ability to neutralize IFN‑ alpha / beta  R1-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R1 Antibody. View Larger

Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R1 Antibody. Recombinant Mouse Limitin (Catalog # 1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IFN-a/ beta R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3039). The ND50 is typically 1-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R1

IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1-3). IFN-alpha / beta R1, in association with IFN-alpha / beta R2, is required for propagating antiviral signal transduction triggered by IFN-alpha and IFN-beta (4, 5). The mouse IFN-alpha / beta R1 cDNA encodes a 590 amino acid (aa) precursor including a 26 aa signal sequence, a 403 aa extracellular domain (ECD), a 20 aa transmembrane segment, and a 141 aa cytoplasmic domain (6). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. The ECD of mouse IFN-alpha / beta R1 shares 47-48% aa identity with that of human, bovine, porcine, and ovine IFN-alpha / beta R1. IFN-alpha / beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN-alpha / beta R2. Ligands associate with IFN-alpha / beta R2, and this complex subsequently forms a stable ternary assembly with IFN-alpha / beta R1 (7, 8). IFN-alpha / beta R1 also associates with IFN-gamma R2 even in the absence of IFN-gamma stimulation (5). Tyrosine phosphorylation within the juxtamembrane cytoplasmic domain of IFN-alpha / beta R1 provides a docking site for the SH2 domains of Tyk2 and STAT2 (9-11). Tyk2 can directly phosphorylate IFN-alpha / beta R1 (10). Tyk2 also increases the level of cell surface expression of IFN-alpha / beta R1 by preventing constitutive internalization (12). Human IFN-alpha / beta R1 contains a nuclear localization signal in its ECD which is required for receptor translocation to the nucleus following interaction with ligand (13).

References
  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Kotenko, S.V. and J.A. Langer (2004) Int. Immunopharmacol. 4:593.
  3. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  4. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284.
  5. Takaoka, A. et al. (2000) Science 288:2357.
  6. Uze, G. et al. (1992) Proc. Natl. Acad. Sci. USA 89:4774.
  7. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
  8. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
  9. Krishnan, K. et al. (1998) J. Biol. Chem. 273:19495.
  10. Yan, H. et al. (1996) Mol. Cell. Biol. 16:2074.
  11. Yan, H. et al. (1996) EMBO J. 15:1064.
  12. Ragimbeau, J. et al. (2003) EMBO J. 22:537.
  13. Subramaniam, P.S. and H.M. Johnson (2004) FEBS Lett. 578:207.
Long Name
Interferon alpha/beta Receptor 1
Entrez Gene IDs
3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)
Alternate Names
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1

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Citations for Mouse IFN-alpha / beta  R1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Hes1 attenuates type I IFN responses via VEGF-C and WDFY1
    Authors: Ning F, Li X, Yu L et al.
    Journal of Experimental Medicine
  2. UBXN3B Controls Immunopathogenesis of Arthritogenic Alphaviruses by Maintaining Hematopoietic Homeostasis
    Authors: T Geng, D Yang, T Lin, JG Cahoon, P Wang
    MBio, 2022-11-15;0(0):e0268722.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Hes1 attenuates type I IFN responses via VEGF-C and WDFY1
    Authors: Ning F, Li X, Yu L et al.
    Journal of Experimental Medicine
  4. Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells.
    Authors: Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F
    Immunol. Lett., 2007-12-17;116(1):86-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Toll-like receptor agonist combinations augment mouse T-cell anti-tumor immunity via IL-12- and interferon beta -mediated suppression of immune checkpoint receptor expression
    Authors: Donghwan Jeon, Douglas G. McNeel
    OncoImmunology

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