Mouse IgD Antibody

Catalog #: MAB9858 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9858-100
MAB9858-SP

Detection of IgD in Mouse Splenocytes by Flow Cytometry

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All IgD Antibodies

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Mouse IgD Antibody Summary

Species Reactivity

Mouse

Specificity

Reacts with the delta chain of Mouse IgD of all haplotypes and does not cross-react with other immunoglobulin isotypes.

Source

Monoclonal Rat IgG_2A Clone # 11-26C

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse IgD

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IgD in Mouse Splenocytes by Flow Cytometry Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse IgD Monoclonal Antibody (Catalog # MAB9858) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgD

Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.

Long Name

Immunoglobulin D

Entrez Gene IDs

3495 (Human); 380797 (Mouse)

Alternate Names

IgD; Igh-5; Ighd; Immunoglobulin D; Immunoglobulin Delta