Home / IGF-II/IGF2 / Mouse IGF-II/IGF2 DuoSet ELISA

Mouse IGF-II/IGF2 DuoSet ELISA

Catalog #: DY792
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY792
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
DY009B: DuoSet ELISA Ancillary Reagent Kit 3
Mouse IGF-II ELISA Standard Curve
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Product Details
Procedure
Citations (10)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IGF-II/IGF2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IGF-II. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 3 (5 plates): (Catalog # DY009) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 3.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY004), or 5% Tween 20 in PBS, 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

 

Scientific Data

N/A Mouse IGF-II ELISA Standard Curve View Larger

Mouse IGF-II ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-II/IGF2

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

Long Name:
Insulin-like Growth Factor II
Entrez Gene IDs:
3481 (Human); 16002 (Mouse)
Alternate Names:
C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A

Assay Procedure

GENERAL ELISA PROTOCOL


Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL of Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IGF-II/IGF2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice
    Authors: Sandovici, I;Fernandez-Twinn, DS;Campbell, N;Cooper, WN;Sekita, Y;Zvetkova, I;Ferland-McCollough, D;Prosser, HM;Oyama, LM;Pantaleão, LC;Cimadomo, D;Barbosa de Queiroz, K;Cheuk, CSK;Smith, NM;Kay, RG;Antrobus, R;Hoelle, K;Ma, MKL;Smith, NH;Geyer, SH;Reissig, LF;Weninger, WJ;Siddle, K;Willis, AE;Lam, BYH;Bushell, M;Ozanne, SE;Constância, M;
    Cell reports
    Species: Mouse, Transgenic Mouse
    Sample Types: Tissue Homogenates
  2. Mesenchyme-derived IGF2 is a major paracrine regulator of pancreatic growth and function
    Authors: CM Hammerle, I Sandovici, GV Brierley, NM Smith, WE Zimmer, I Zvetkova, HM Prosser, Y Sekita, BYH Lam, M Ma, WN Cooper, A Vidal-Puig, SE Ozanne, G Medina-Góm, M Constância
    PLoS Genet, 2020-10-15;16(10):e1009069.
    Species: Mouse
    Sample Types: Cell Lysates
  3. Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice
    Authors: Y Sui, W Zhang, T Tang, L Gao, T Cao, H Zhu, Q You, B Yu, T Yang
    Stem Cell Res Ther, 2020-02-26;11(1):86.
    Species: Mouse
    Sample Types: Serum
  4. Immune targeting of autocrine IGF2 hampers rhabdomyosarcoma growth and metastasis
    Authors: C De Giovann, P Nanni, L Landuzzi, ML Ianzano, G Nicoletti, S Croci, A Palladini, PL Lollini
    BMC Cancer, 2019-02-07;19(1):126.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1
    Authors: N Dai, F Ji, J Wright, L Minichiell, RI Sadreyev, J Avruch
    Elife, 2017-07-28;6(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis.
    Authors: Han Y, Cui J, Tao J, Guo L, Guo P, Sun M, Kang J, Zhang X, Yan C, Li S
    Exp. Cell Res., 2009-09-19;315(19):3301-11.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice.
    Authors: Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA
    Stem Cells, 2008-05-15;26(7):1778-86.
    Species: Mouse
    Sample Types: Plasma
  8. Forkhead box protein O1 negatively regulates skeletal myocyte differentiation through degradation of mammalian target of rapamycin pathway components.
    Authors: Wu AL, Kim JH, Zhang C, Unterman TG, Chen J
    Endocrinology, 2007-12-13;149(3):1407-14.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion.
    Authors: Wanderling S, Simen BB, Ostrovsky O, Ahmed NT, Vogen SM, Gidalevitz T, Argon Y
    Mol. Biol. Cell, 2007-07-18;18(10):3764-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Role of the forkhead protein FoxO1 in beta cell compensation to insulin resistance.
    Authors: Okamoto H, Hribal ML, Lin HV, Bennett WR, Ward A, Accili D
    J. Clin. Invest., 2006-02-16;116(3):775-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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