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Mouse IgG1 Alexa Fluor® 405-conjugated Antibody

Catalog #: IC002V
2 Citations Datasheet / COA / SDS
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IC002V
R&D Systems Antibodies
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Citations (2)
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Mouse IgG1 Alexa Fluor® 405-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

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Citations for Mouse IgG1 Alexa Fluor® 405-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Senescent mesenchymal stem/stromal cells in pre-metastatic bone marrow of untreated advanced breast cancer patients
    Authors: Francisco Raúl RA贚 BORZONE, María Belén BEL蒒 GIORELLO, Leandro Marcelo MARCELO MARTINEZ, María Cecilia CECILIA SANMARTIN, LEONARDO FELDMAN, FEDERICO DIMASE et al.
    Oncology Research
  2. Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis
    Authors: B Pang, Y Zhu, J Ni, J Ruan, J Thompson, D Malouf, J Bucci, P Graham, Y Li
    International Journal of Nanomedicine, 2020-12-15;15(0):10241-10256.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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