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Mouse IgG1 APC-conjugated Antibody

Catalog #: IC002A
44 Citations 4 Reviews Datasheet / COA / SDS
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IC002A
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Detection of Mouse IgG Isotype Control by Flow Cytometry
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Citations (44)
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Reviews (4)
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Mouse IgG1 APC-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood monocytes were stained with Mouse Anti-Human LILRA6/CD85b APC-conjugated Monoclonal Antibody (Catalog # FAB8656A, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

Product Datasheets

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Citations for Mouse IgG1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair
    Authors: T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton
    Cell Rep, 2019-03-05;26(10):2566-2579.e10.
  2. Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors
    Authors: FJ Zapatero-B, E Dietzel, O Dolnik, K Döhner, R Costa, B Hertel, B Veselkova, J Kirui, A Klintworth, MP Manns, S Pöhlmann, T Pietschman, T Krey, S Ciesek, G Gerold, B Sodeik, S Becker, T von Hahn
    Viruses, 2019-03-19;11(3):.
  3. GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis
    Authors: Bisson, JA;Gordillo, M;Kumar, R;de Silva, N;Yang, E;Banks, KM;Shi, ZD;Lee, K;Yang, D;Chung, WK;Huangfu, D;Evans, T;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  4. MET-induced CD73 restrains STING-mediated immunogenicity of EGFR-mutant lung cancer
    Authors: R Yoshida, M Saigi, T Tani, BF Springer, H Shibata, S Kitajima, NR Mahadevan, M Campisi, W Kim, Y Kobayashi, TC Thai, K Haratani, Y Yamamoto, SK Sundararam, EH Knelson, A Vajdi, I Canadas, R Uppaluri, CP Paweletz, JJ Miret, PH Lizotte, PC Gokhale, PA Janne, DA Barbie
    Cancer Research, 2022-11-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  5. Functional Downregulation of PD-L1 and PD-L2 by CpG and non-CpG Oligonucleotides in Melanoma Cells
    Authors: J Kleemann, K Steinhorst, V König, N Zöller, J Cinatl, D Özistanbul, R Kaufmann, M Meissner, S Kippenberg
    Cancers, 2022-09-27;14(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Cargo and Functional Profile of Saliva-Derived Exosomes Reveal Biomarkers Specific for Head and Neck Cancer
    Authors: Linda Hofmann, Valentin Medyany, Jasmin Ezić, Ramin Lotfi, Beate Niesler, Ralph Röth et al.
    Frontiers in Medicine
  7. Integrative analysis reveals a lineage-specific circular RNA landscape for adipo-osteogenesis of human mesenchymal stem cells
    Authors: Hai-Bo Huang, Hai-Tao Luo, Na-Na Wei, Miao-Ling Liu, Fei He, Wei Yang et al.
    Stem Cell Research & Therapy
  8. Impact of Extracellular pH on Apoptotic and Non-Apoptotic TRAIL-Induced Signaling in Pancreatic Ductal Adenocarcinoma Cells
    Authors: Sofie Hagelund, Anna Trauzold
    Frontiers in Cell and Developmental Biology
  9. Dual Inhibition of Myc Transcription and PI3K Activity Effectively Targets Colorectal Cancer Stem Cells
    Authors: Miriam Gaggianesi, Laura Rosa Mangiapane, Chiara Modica, Vincenzo Davide Pantina, Gaetana Porcelli, Simone Di Franco et al.
    Cancers (Basel)
  10. Decrease of Pro-Angiogenic Monocytes Predicts Clinical Response to Anti-Angiogenic Treatment in Patients with Metastatic Renal Cell Carcinoma
    Authors: Stephane Oudard, Nadine Benhamouda, Bernard Escudier, Patrice Ravel, Thi Tran, Emeline Levionnois et al.
    Cells
  11. TGF-beta 1 potentiates V gamma 9Vδ2 T cell adoptive immunotherapy of cancer
    Authors: Richard E. Beatson, Ana C. Parente-Pereira, Leena Halim, Domenico Cozzetto, Caroline Hull, Lynsey M. Whilding et al.
    Cell Reports Medicine
  12. Nobiletin and Xanthohumol Sensitize Colorectal Cancer Stem Cells to Standard Chemotherapy
    Authors: A Turdo, A Glaviano, G Pepe, F Calapà, S Raimondo, ME Fiori, D Carbone, MG Basilicata, V Di Sarno, C Ostacolo, B Parrino, S Cascioferr, C Pecoraro, S Di Franco, D Bellavia, M Gaggianesi, V Veschi, M Lo Iacono, G Ganduscio, VD Pantina, LR Mangiapane, MR Bongiorno, R Alessandro, M Todaro, R De Maria, P Diana, P Campiglia, G Stassi
    Cancers, 2021-08-04;13(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. CHK1 inhibitor sensitizes resistant colorectal cancer stem cells to nortopsentin
    Authors: Simone Di Franco, Barbara Parrino, Miriam Gaggianesi, Vincenzo Davide Pantina, Paola Bianca, Annalisa Nicotra et al.
    iScience
  14. Magnetic Nanoparticle-Based Hyperthermia Mediates Drug Delivery and Impairs the Tumorigenic Capacity of Quiescent Colorectal Cancer Stem Cells
    Authors: Soraia Fernandes, Tamara Fernandez, Sabrina Metze, Preethi B. Balakrishnan, Binh T. Mai, John Conteh et al.
    ACS Applied Materials & Interfaces
  15. Two 20-Residue-Long Peptides Derived from Plasmodium vivax Merozoite Surface Protein 10 EGF-Like Domains Are Involved in Binding to Human Reticulocytes
    Authors: LA Ricaurte-C, A Lovera, DA Moreno-Pér, MD Bohórquez, CF Suárez, E Gutiérrez-, L Cuy-Chapar, D Garzón-Osp, MA Patarroyo
    International Journal of Molecular Sciences, 2021-02-05;22(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Targeting the ABC transporter ABCB5 sensitizes glioblastoma to temozolomide-induced apoptosis through a cell-cycle checkpoint regulation mechanism
    Authors: CAA Lee, P Banerjee, BJ Wilson, S Wu, Q Guo, G Berg, S Karpova, A Mishra, JW Lian, J Tran, M Emmerich, GF Murphy, MH Frank, NY Frank
    J. Biol. Chem., 2020-04-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Development of an ex vivo xenogeneic bone environment producing human platelet-like cells
    Authors: S Fujiyama, N Hori, T Sato, S Enosawa, M Murata, E Kobayashi
    PLoS ONE, 2020-04-07;15(4):e0230507.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Increase in bone metabolic markers and circulating osteoblast-lineage cells after orthognathic surgery
    Authors: Y Abe, M Chiba, S Yaklai, RS Pechayco, H Suzuki, T Takahashi
    Sci Rep, 2019-12-27;9(1):20106.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Infrapatellar Fat Pad Stem Cells Responsiveness to Microenvironment in Osteoarthritis: From Morphology to Function
    Authors: Elena Stocco, Silvia Barbon, Monica Piccione, Elisa Belluzzi, Lucia Petrelli, Assunta Pozzuoli et al.
    Frontiers in Cell and Developmental Biology
  20. Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR
    Authors: W Nowak, LN Grendas, LM Sanmarco, IG Estecho, ÁR Arena, N Eberhardt, DE Rodante, MP Aoki, FM Daray, EA Carrera Si, AE Errasti
    EBioMedicine, 2019-11-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. CCR5 receptor antagonism inhibits hepatitis C virus (HCV) replication in vitro
    Authors: Jason T. Blackard, Ling Kong, Susan D. Rouster, Rebekah Karns, Paul S. Horn, Shyam Kottilil et al.
    PLOS ONE
  22. Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant
    Authors: V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi
    Oncogene, 2019-10-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. TRAIL Induces Nuclear Translocation and Chromatin Localization of TRAIL Death Receptors
    Authors: U Mert, A Adawy, E Scharff, P Teichmann, A Willms, V Haselmann, C Colmorgen, J Lemke, S von Karste, J Fritsch, A Trauzold
    Cancers (Basel), 2019-08-14;11(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Identification of LPS-Activated Endothelial Subpopulations With Distinct Inflammatory Phenotypes and Regulatory Signaling Mechanisms
    Authors: Erna-Zulaikha Dayang, Josée Plantinga, Bram ter Ellen, Matijs van Meurs, Grietje Molema, Jill Moser
    Frontiers in Immunology
  25. Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells
    Authors: A Willms, H Schittek, S Rahn, J Sosna, U Mert, D Adam, A Trauzold
    PLoS ONE, 2019-04-04;14(4):e0214847.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Induction of MET Receptor Tyrosine Kinase Down-regulation through Antibody-mediated Receptor Clustering
    Authors: W Li, A Dick, F Lu, H Zhang, H Sun
    Sci Rep, 2019-02-13;9(1):1988.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. CXCR7 expression in diffuse large B-cell lymphoma identifies a subgroup of CXCR4+ patients with good prognosis
    Authors: MJ Moreno, A Gallardo, S Novelli, A Mozos, M Aragó, MÁ Pavón, MV Céspedes, V Pallarès, A Falgàs, M Alcoceba, O Blanco, M Gonzalez-D, J Sierra, R Mangues, I Casanova
    PLoS ONE, 2018-06-19;13(6):e0198789.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor
    Authors: WJ Zacharias, DB Frank, JA Zepp, MP Morley, FA Alkhaleel, J Kong, S Zhou, E Cantu, EE Morrisey
    Nature, 2018-02-28;555(7695):251-255.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  29. Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R
    Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He
    J. Cell. Mol. Med., 2017-09-18;0(0):.
    Applications: Flow Cytometry Control
  30. Equine Mesenchymal Stromal Cells retain a pericyte-like phenotype
    Authors: CL Esteves, TA Sheldrake, L Dawson, T Menghini, E Rink, K Amilon, N Khan, B Peault, X Donadeu
    Stem Cells Dev., 2017-05-09;0(0):.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Systemic frequencies of T helper 1 and T helper 17 cells in patients with age-related macular degeneration: A case-control study
    Authors: Amardeep Singh, Yousif Subhi, Marie Krogh Nielsen, Mads Krüger Falk, Sara Maj Hyldig Matzen, Finn Sellebjerg et al.
    Scientific Reports
  32. Biological Activity of Masked Endotoxin
    Authors: H Schwarz, J Gornicec, T Neuper, MA Parigiani, M Wallner, A Duschl, J Horejs-Hoe
    Sci Rep, 2017-03-20;7(0):44750.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  33. Differential cytokine contributions of perivascular haematopoietic stem cell niches
    Authors: N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette
    Nat. Cell Biol, 2017-02-20;19(3):214-223.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. CHIR99021 combined with retinoic acid promotes the differentiation of primordial germ cells from human embryonic stem cells
    Authors: Tingting Cheng, Kui Zhai, Yan Chang, Guidong Yao, Jiahuan He, Fang Wang et al.
    Oncotarget
  35. Hepatitis C Virus Activates a Neuregulin-Driven Circuit to Modify Surface Expression of Growth Factor Receptors of the ErbB Family
    Authors: Sabine Stindt, Patricia Cebula, Ute Albrecht, Verena Keitel, Jan Schulte am Esch, Wolfram T. Knoefel et al.
    PLOS ONE
  36. Prerequisites for Functional Interleukin 31 Signaling and Its Feedback Regulation by Suppressor of Cytokine Signaling 3 (SOCS3).
    Authors: Maier E, Mittermeir M, Ess S, Neuper T, Schmiedlechner A, Duschl A, Horejs-Hoeck J
    J Biol Chem, 2015-08-25;290(41):24747-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. Notch3 functions as a regulator of cell self-renewal by interacting with the beta -catenin pathway in hepatocellular carcinoma
    Authors: Qingyu Zhang, Caijie Lu, Tao Fang, Yongcun Wang, Wenhua Hu, Jie Qiao et al.
    Oncotarget
  38. Signaling via PI3K/FOXO1A pathway modulates formation and survival of human embryonic stem cell-derived endothelial cells.
    Authors: Merkely B, Gara E, Lendvai Z, Skopal J, Leja T, Zhou W, Kosztin A, Varady G, Mioulane M, Bagyura Z, Nemeth T, Harding S, Foldes G
    Stem Cells Dev, 2014-12-22;24(7):869-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. Cancer stem-like sphere cells induced from de-differentiated hepatocellular carcinoma-derived cell lines possess the resistance to anti-cancer drugs.
    Authors: Hashimoto, Noriaki, Tsunedomi, Ryouichi, Yoshimura, Kiyoshi, Watanabe, Yusaku, Hazama, Shoichi, Oka, Masaaki
    BMC Cancer, 2014-09-27;14(0):722.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  40. The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.
    Authors: Hoellenriegel J, Zboralski D, Maasch C, Rosin N, Wierda W, Keating M, Kruschinski A, Burger J
    Blood, 2013-11-25;123(7):1032-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. Loss of Siglec-14 reduces the risk of chronic obstructive pulmonary disease exacerbation
    Authors: Takashi Angata, Takeo Ishii, Takashi Motegi, Ritsuko Oka, Rachel E. Taylor, Paula Campos Soto et al.
    Cellular and Molecular Life Sciences
  42. VEGFR2 expressing circulating (progenitor) cell populations in volunteers and cancer patients.
    Authors: Vroling L, Yuana Y, Schuurhuis GJ, van Hinsbergh VW, Gundy C, de Haas R, van Cruijsen H, Boven E, Hoekman K, Broxterman HJ
    Thromb. Haemost., 2007-08-01;98(2):440-50.
    Species: Human
    Sample Types: Whole Blood
    Applications: Control
  43. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006-05-11;126(9):2096-105.
    Species: Human
    Sample Types: Serum
    Applications: Control
  44. Changes in circulating exosome molecular profiles following surgery/(chemo)radiotherapy: early detection of response in head and neck cancer patients
    Authors: MN Theodoraki, S Laban, EK Jackson, R Lotfi, PJ Schuler, C Brunner, TK Hoffmann, TL Whiteside, L Hofmann
    British Journal of Cancer, 2021-10-12;0(0):.

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Reviews for Mouse IgG1 APC-conjugated Antibody

Average Rating: 4.5 (Based on 4 Reviews)

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Mouse IgG1 APC-conjugated Antibody
By Anonymous on 08/11/2024
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse
Flow Cytometry Mouse IgG1 APC-conjugated Antibody IC002A
Mouse IgG1 APC-conjugated Antibody
By Abby Sukarto on 02/14/2020
Application: Flow Sample Tested: Bone Extracts Species: Human
Flow Cytometry Mouse IgG1 APC-conjugated Antibody IC002A
Mouse IgG1 APC-conjugated Antibody
By Anonymous on 09/10/2016
Application: Flow Sample Tested: ASTROCYTE Species: Human
Flow Cytometry Mouse IgG1 APC-conjugated Antibody IC002A
Mouse IgG1 APC-conjugated Antibody
By Anonymous on 06/15/2016
Application: Flow Sample Tested: Human embryonic stem cells derived mesoderm (H1) Species: Human

1 million of H1 embryonic stem cells were incubated with 10ul of mouse IgG1 APC-conjugated and 10 ul of mouse IgG1 PE-conjugated Antibodies for 45min at 4C in dark. Cells were analyzed user the LSRII analyzer.

Flow Cytometry Mouse IgG1 APC-conjugated Antibody IC002A