Mouse IgG_2A Alexa Fluor® 700-conjugated Antibody

Catalog #: IC003N
1 Citation Datasheet / COA / SDS

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IC003N

Detection of Mouse IgG2AIsotype Control by Flow Cytometry

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Mouse IgG_2A Alexa Fluor® 700-conjugated Antibody Summary

Specificity

Selected for use as mouse IgG_2A isotype control for flow cytometry applications.

Source

Monoclonal Mouse IgG_2A Clone # 20102

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

KLH

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/10⁶ cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control

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Detection of Mouse IgG_2AControl by Flow Cytometry Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB330N, filled histogram) or Mouse IgG Alexa Fluor® 700-conjugated Isotype Control Antibody (Catalog # IC003N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Alternate Names

IgG2A

Product Datasheets

Product Datasheet

COA

SDS

Citation for Mouse IgG_2A Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1