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Mouse IgG2B PE-conjugated Antibody

Catalog #: FAB9636P Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB9636P-100
Detection of Mouse IgG2B PrimaryAntibody by Anti-Mouse IgG2B-specific Secondary Antibody.
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Mouse IgG2B PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG2B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with mouse IgG1, mouse IgG2A, and mouse IgG3 was observed.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2215A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mouse IgG2B PrimaryAntibody by Anti-Mouse IgG2B-specific Secondary Antibody. View Larger

Detection of Mouse IgG2B PrimaryAntibody by Anti-Mouse IgG2B-specific Secondary Antibody. Human PBMC lymphocytes were stained with Mouse anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B isotype, filled histogram) or Mouse anti-HumanCD3 epsilon Monoclonal Antibody (Catalog # MAB100, Mouse IgG1 isotype, open histogram), followed by Rabbit anti-Mouse IgG2B-specific PE-conjugated secondary antibody (Catalog #FAB9636P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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