Home / IgG / Mouse IgG NorthernLights™ NL637-conjugated Antibody

Mouse IgG NorthernLights™ NL637-conjugated Antibody

Catalog #: NL008
15 Citations Datasheet / COA / SDS
NorthernLights 637 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL008
Detection of Mouse IgG Primary Antibody by Immunocytochemistry.
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Product Details
Citations (15)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG NorthernLights™ NL637-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Mouse IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL637 (Excitation= 637 nm, Emission= 658 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Use at a 1:200 dilution
 
Immunohistochemistry
Use at a 1:200 dilution
 
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Mouse IgG Primary Antibody by Immunocytochemistry. Detection of human Vimentin by incubating cells with 8 μg/mL of Mouse anti-Human Vimentin monoclonal antibody (MAB21052) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Mouse IgG NorthernLights™ NL637-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. CDH2 mutation affecting N-cadherin function causes attention-deficit hyperactivity disorder in humans and mice
    Authors: D Halperin, A Stavsky, R Kadir, M Drabkin, O Wormser, Y Yogev, V Dolgin, R Proskorovs, Y Perez, H Nudelman, O Stoler, B Rotblat, T Lifschytz, A Lotan, G Meiri, D Gitler, OS Birk
    Nature Communications, 2021-10-26;12(1):6187.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Opposite Control of Excitatory and Inhibitory Synapse Formation by Slitrk2 and Slitrk5 on Dopamine Neurons Modulates Hyperactivity Behavior
    Authors: C Salesse, J Charest, H Doucet-Bea, AM Castonguay, S Labrecque, P De Koninck, M Lévesque
    Cell Rep, 2020-02-18;30(7):2374-2386.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Maximizing Explanatory Power in Stereological Data Collection: A Protocol for Reliably Integrating Optical Fractionator and Multiple Immunofluorescence Techniques
    Authors: Anna Kreutz, Nicole Barger
    Frontiers in Neuroanatomy
  4. Evidence for Homodimerization of the c-Fos Transcription Factor in Live Cells Revealed by Fluorescence Microscopy and Computer Modeling.
    Authors: Szaloki N, Krieger J, Komaromi I, Toth K, Vamosi G
    Mol Cell Biol, 2015-08-24;35(21):3785-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  5. Clinical improvement in psoriasis with specific targeting of interleukin-23.
    Authors: Kopp T, Riedl E, Bangert C, Bowman E, Greisenegger E, Horowitz A, Kittler H, Blumenschein W, McClanahan T, Marbury T, Zachariae C, Xu D, Hou X, Mehta A, Zandvliet A, Montgomery D, van Aarle F, Khalilieh S
    Nature, 2015-03-09;521(7551):222-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons.
    Authors: Shulman Y, Stavsky A, Fedorova T, Mikulincer D, Atias M, Radinsky I, Kahn J, Slutsky I, Gitler D
    J Neurosci, 2015-01-21;35(3):985-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  7. Abluminal Stimulation of Sphingosine 1-Phosphate Receptors 1 and 3 Promotes and Stabilizes Endothelial Sprout Formation
    Authors: Anusuya Das, Steven M. Lenz, Anthony O. Awojoodu, Edward A. Botchwey
    Tissue Engineering Part A
  8. 1,25-Dihydroxyvitamin D(3) Inhibits Podocyte uPAR Expression and Reduces Proteinuria
    Authors: Jianchao Ma, Bin Zhang, Shuangxin Liu, Shaoting Xie, Yun Yang, Juan Ma et al.
    PLoS ONE
  9. The calcineurin–NFAT pathway allows for urokinase receptor-mediated beta3 integrin signaling to cause podocyte injury
    Authors: Bin Zhang, Wei Shi, Juan Ma, Alexis Sloan, Christian Faul, Changli Wei et al.
    Journal of Molecular Medicine
  10. Phenotypic characterization of leukocytes in prenatal human dermis.
    J. Invest. Dermatol., 2012-06-21;132(11):2581-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
    Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
    Lab. Invest., 2012-03-26;92(7):967-77.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Nuclear Receptor HNF4 alpha Binding Sequences are Widespread in Alu Repeats
    Authors: Eugene Bolotin, Karthikeyani Chellappa, Wendy Hwang-Verslues, Jake M Schnabl, Chuhu Yang, Frances M Sladek
    BMC Genomics
  13. Neisseria meningitidis adhesin NadA targets beta1 integrins: functional similarity to Yersinia invasin.
    Authors: Nagele V, Heesemann J, Schielke S, Jimenez-Soto LF, Kurzai O, Ackermann N
    J. Biol. Chem., 2011-04-06;286(23):20536-46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Developmental guidance of embryonic corneal innervation: roles of Semaphorin3A and Slit2.
    Authors: Kubilus JK, Linsenmayer TF
    Dev. Biol., 2010-05-18;344(1):172-84.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  15. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009-01-12;206(1):169-81.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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