Mouse IkB-epsilon Antibody

Catalog #: AF4637
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4637
AF4637-SP

Detection of Mouse IkB-epsilon by Western Blot.

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All IkB-epsilon Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IkB-epsilon Antibody Summary

Species Reactivity

Mouse

Specificity

Detects endogenous mouse IkB-epsilon in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse I kappa B-epsilon
Met1-Asp364
Accession # O54910

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse IkB-epsilon by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 1 µg/mL of Mouse IkB-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4637) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IkB-e at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IkB-epsilon

NF kappa B inhibitor epsilon (IkB-epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Long Name

I-kappa-B-epsilon

Entrez Gene IDs

4794 (Human); 18037 (Mouse)

Alternate Names

epsilon; ikappaBepsilon; I-kappa-B-epsilon; IkB-E; IKBENF-kappa-B inhibitor epsilon; IkBepsilon; IkB-epsilon; NF-kappa-BIE; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse IkB-epsilon Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1