Mouse IL-10 R beta APC-conjugated Antibody

Catalog #: FAB53681A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB53681A-100UG

Detection of IL‑10 R beta in EL4.IL-2 Mouse Cell Line by Flow Cytometry.

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All IL-10 R beta Antibodies

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Mouse IL-10 R beta APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-10 R beta in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 547324

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-10 R beta
Ile21-Ser220
Accession # Q61190

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑10 R beta in EL4.IL-2 Mouse Cell Line by Flow Cytometry. EL4.IL-2 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL-10 R beta APC-conjugated Monoclonal Antibody (Catalog # FAB53681A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-10 R beta

IL-10 R beta (Interleukin 10 receptor beta; also IL-10R2, CD210b and CRF2-4) is an 80-85 kDa member of the type II cytokine receptor family of proteins. It is very widely expressed and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B, and -29 (plus IL-26 in human). Mature mouse IL-10 R beta is a type I transmembrane protein with an extracellular domain that possesses two fibronectin type III domains (aa 22-107 and 111‑208). There is one alternate start site at Met20. Over aa 21- 220, mouse IL-10 R beta shares 87% and 74% aa identity with rat and human IL-10 R beta, respectively.

Long Name

Interleukin 10 Receptor beta

Entrez Gene IDs

3588 (Human); 16155 (Mouse)

Alternate Names

CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 R beta; IL-10 receptor subunit beta; IL10R beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10Rb; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4