Mouse IL-11 R alpha Antibody

Catalog #: AF490
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF490
AF490-SP

Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 R alpha Antibody.

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Citations (3)

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Mouse IL-11 R alpha Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-11 R alpha in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-11 R alpha
Ser22-Gln367
Accession # Q64385

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑11 R alpha Fc Chimera (Catalog # 490-IR)

Neutralization

Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND₅₀) is typically 1-6 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑11.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 R alpha Antibody. Recombinant Mouse IL-11 (Catalog # 418-ML) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-11 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-11 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF490). The ND₅₀ is typically 1-6 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-11 R alpha

Mouse interleukin 11 receptor alpha (IL-11 R alpha ), originally designated NR1, is a member of the hematopoietic cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 432 amino acid (aa) residue precursor type I membrane protein with a 23 aa residue signal peptide, a 344 aa residue extracellular domain containing two potential glycosylation sites, a 26 aa residue transmembrane region and a short (39 aa residue) cytoplasmic domain. In comparison with other hematopoietic cytokine receptors, the extracellular domain of IL-11 R alpha is closely related to that of IL-6 R alpha, CNTF R alpha and the p40 subunit of IL‑12, exhibiting 24%, 22% and 16% amino acid sequence identity, respectively. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha, together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist.

References

Taga, T. and T. Kishimoto (1997) Annu. Rev. Immuno. 15:797.
Hilton, D. et al. (1994) EMBO J. 13:4765.
Davidson, A.J. et al. (1997) Stem Cells 15:119.
Curtis, D.J. et al. (1997) Blood 90:4403.

Long Name

Interleukin 11 Receptor alpha

Entrez Gene IDs

3590 (Human); 16157 (Mouse)

Alternate Names

AI314697; GP130; IL-11 R alpha; IL11R alpha; IL11RA; IL-11Ra; Il11ra2; NR1

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Related Research Areas

Citations for Mouse IL-11 R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Interleukin-11 regulates the fate of adipose-derived mesenchymal stem cells via STAT3 signalling pathways
Authors: Wenlong Yang, Shuning Zhang, Tiantong Ou, Hao Jiang, Daile Jia, Zhiyong Qi et al.
Cell Proliferation
Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells
Authors: K Murata, U Jadhav, S Madha, J van Es, J Dean, A Cavazza, K Wucherpfen, F Michor, H Clevers, RA Shivdasani
Cell Stem Cell, 2020-02-20;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC/IF
Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
Cell. Signal., 2008-10-12;21(1):151-60.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot