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Mouse IL-12 Biotinylated Antibody

Catalog #: BAF419
10 Citations 1 Review Datasheet / COA / SDS
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BAF419
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-12 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-12 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse IL‑12 p40, recombinant human (rh) IL-23, and rhIL-23 p40 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12
p40 (Met23-Ser335, P43432) p35 (Arg23-Ala215, NP_032377)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑12 (Catalog # 419-ML)

Mouse IL-12 p70 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IL‑12 p70 Antibody (Catalog # MAB419)

Standard: Recombinant Mouse IL-12 Protein (Catalog # 419-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

Long Name
Interleukin 12
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)
Alternate Names
CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

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Citations for Mouse IL-12 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction
    Authors: J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss
    The Journal of Biological Chemistry, 2021-10-09;0(0):101295.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
    Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
    J. Allergy Clin. Immunol., 2016-10-26;139(5):1608-1620.e3.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  3. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  4. IL-35 stimulation of CD39+ regulatory T cells confers protection against collagen II-induced arthritis via the production of IL-10.
    Authors: Kochetkova I, Golden S, Holderness K
    J. Immunol., 2010-05-10;184(12):7144-53.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  5. Expression of bioactive single-chain murine IL-12 in transgenic plants.
    Authors: Liu J, Dolan MC, Reidy M, Cramer CL
    J. Interferon Cytokine Res., 2008-06-01;28(0):381-92.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  6. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006-12-15;177(12):8650-7.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
    Applications: ELISA Development
  7. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006-06-01;168(6):1940-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  8. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
    Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
    J Ethnopharmacol, 2006-03-10;106(3):418-24.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  9. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  10. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004-05-13;104(5):1404-10.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Mouse IL-12 Biotinylated Antibody
By Anonymous on 11/27/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Mouse

This antibody was paired with MAB419 to create an ELISA for mouse IL-12.

ELISA Mouse IL-12 Biotinylated Antibody BAF419