Mouse IL-13 Antibody

Catalog #: AB-413-NA Datasheet / COA / SDS

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AB-413-NA

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody.

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Mouse IL-13 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑13 in direct ELISAs and Western blots. In direct ELISAs and Westerns, less than 1% cross-reactivity with recombinant human IL-13 is observed. Neutralizes the biological activity of recombinant mouse IL-13, but will not neutralize the biological activity of recombinant human IL-13.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse IL-13
Ser26-Phe131
Accession # P20109

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑13 (Catalog # 413-ML)

Neutralization

Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 15-30 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL-13 (Catalog # 413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Polyclonal Antibody (Catalog # AB-413-NA). The ND₅₀ is typically 15-30 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

References