Mouse IL-13 Antibody

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL-13 (413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND₅₀ is typically 0.05-0.15 µg/mL.

IL‑13 in Mouse Splenocytes. IL-13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse IL-13 by Immunohistochemistry Histological lung sections of a p66Shc−/− mouse at 7 months after CS exposure.(A) Positive immunostaining for MAC-3 confirms that the free alveolar cells are predominantly macrophages. A small number of macrophages are also present in the peribronchiolar infiltrates. (B) Representative lung section showing alveolar multinucleated macrophages containing fine granular brown cytoplasmic particles that stain with Perls’ Prussian blue (C). (D) The peribronchiolar areas of inflammation are characterized by a large amount of lymphocytes and a small number of pigmented cells that also stain with Perl’s Prussian blue (E). (F) Many fascin positive cells (histiocytes) are present in the peribronchiolar areas. (A) and (F): Scale bars = 80 μm; (B-E): Scale bars = 40 μm. Immunohistochemistry of CD4 (G), IL-4 (H), IL-13 (I), Arginase I (J), Chitinase (K) and iNOS (L) in the lung tissue of p66Shc−/− mice at 7 months after CS exposure. In Fig. 4L, a M1 macrophage (iNOS positive)(arrowhead) is present together iNOS negative macrophages. (G-L): Scale bars = 40 μm. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0119797), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse IL-13 by Immunohistochemistry Histological lung sections of a p66Shc−/− mouse at 7 months after CS exposure.(A) Positive immunostaining for MAC-3 confirms that the free alveolar cells are predominantly macrophages. A small number of macrophages are also present in the peribronchiolar infiltrates. (B) Representative lung section showing alveolar multinucleated macrophages containing fine granular brown cytoplasmic particles that stain with Perls’ Prussian blue (C). (D) The peribronchiolar areas of inflammation are characterized by a large amount of lymphocytes and a small number of pigmented cells that also stain with Perl’s Prussian blue (E). (F) Many fascin positive cells (histiocytes) are present in the peribronchiolar areas. (A) and (F): Scale bars = 80 μm; (B-E): Scale bars = 40 μm. Immunohistochemistry of CD4 (G), IL-4 (H), IL-13 (I), Arginase I (J), Chitinase (K) and iNOS (L) in the lung tissue of p66Shc−/− mice at 7 months after CS exposure. In Fig. 4L, a M1 macrophage (iNOS positive)(arrowhead) is present together iNOS negative macrophages. (G-L): Scale bars = 40 μm. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0119797), licensed under a CC-BY license. Not internally tested by R&D Systems.