Mouse IL-15 Antibody Summary
Asn49-Ser162
Accession # P48346
Applications
Mouse IL-15 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-15
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1 - 3). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11 - 13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
- Grabstein, K. et al. (1994) Science 264:965.
- Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
- Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
- Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. 94:14444.
- Giri, J.G. et al. (1995) EMBO 14:3654.
- Giri, J. et al. (1994) EMBO J. 13:2822.
- Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
- Dubois, S. et al. (2002) Immunity 17:537.
- Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
- Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
- Mortier, E. et al. (2004) J. Immunol. 173:1681.
- Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
- Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Product Datasheets
Citations for Mouse IL-15 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Effect of treatment with conditioned media derived from C2C12 myotube on adipogenesis and lipolysis in 3T3-L1 adipocytes
Authors: K Tamura, N Goto-Inoue, K Miyata, Y Furuichi, NL Fujii, Y Manabe
PLoS ONE, 2020-08-05;15(8):e0237095.
Species: Mouse
Sample Types: Cell Culture Lysates, Cell Lysates
Applications: Western Blot -
Evidence for acute contraction-induced myokine secretion by C2C12 myotubes
Authors: Y Furuichi, Y Manabe, M Takagi, M Aoki, NL Fujii
PLoS ONE, 2018-10-24;13(10):e0206146.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
Authors: Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S
J. Immunol., 2012-09-19;189(9):4237-46.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.
Authors: Watkins SK, Egilmez NK, Suttles J, Stout RD
J. Immunol., 2007-02-01;178(3):1357-62.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse IL-15 Antibody
Average Rating: 2 (Based on 1 Review)
Have you used Mouse IL-15 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
We tried using this as a capture antibody in an ELISA. It gave very little signal as compared to our other analytes.
