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Mouse IL-15 Antibody

Catalog #: AF447
12 Citations Datasheet / COA / SDS
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AF447
AF447-SP
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Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 Antibody.
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Citations (12)
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Mouse IL-15 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-15 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IL‑15 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-15
Asn49-Ser162
Accession # P48346
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑15 (Catalog # 447-ML)
Immunohistochemistry
5-15 µg/mL
See below
Dual RNAscope ISH-IHC
5-15 µg/mL
Immersion fixed paraffin-embedded sections of mouse intestine
Neutralization
Measured by its ability to neutralize IL‑15-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 30 ng/mL Recombinant Mouse IL‑15.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 Antibody. View Larger

Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 Antibody. Recombinant Mouse IL-15 (Catalog # 447-ML) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-15 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF447). The ND50 is typically 0.4-2.4 µg/mL.

Immunohistochemistry IL‑15 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

IL‑15 in Mouse Intestine. IL-15 was detected in immersion fixed frozen sections of mouse intestine (Peyer patch) using Goat Anti-Mouse IL-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

In-situ Hybridization View Larger

Detection of IL‑15 in Mouse Intestine. Formalin-fixed paraffin-embedded tissue sections of mouse intestine were probed for IL-15 mRNA (ACD RNAScope Probe, catalog # 440651; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse IL-15 polyclonal antibody (R&D Systems catalog # AF447) at 1ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

References
  1. Grabstein, K. et al. (1994) Science 264:965.
  2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  5. Giri, J.G. et al. (1995) EMBO 14:3654.
  6. Giri, J. et al. (1994) EMBO J. 13:2822.
  7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  8. Dubois, S. et al. (2002) Immunity 17:537.
  9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
  14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Long Name
Interleukin 15
Entrez Gene IDs
3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

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Citations for Mouse IL-15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Eugenol mimics exercise to promote skeletal muscle fiber remodeling and myokine IL-15 expression by activating TRPV1 channel
    Authors: Huang, T;Chen, X;He, J;Zheng, P;Luo, Y;Wu, A;Yan, H;Yu, B;Chen, D;Huang, Z;
    eLife
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  2. Microwave ablation of primary breast cancer inhibits metastatic progression in model mice via activation of natural killer cells
    Authors: M Yu, H Pan, N Che, L Li, C Wang, Y Wang, G Ma, M Qian, J Liu, M Zheng, H Xie, L Ling, Y Zhao, X Guan, Q Ding, W Zhou, S Wang
    Cell. Mol. Immunol., 2020-05-08;0(0):.
    Species: Mouse
    Sample Types:
    Applications: Neutralization
  3. DeSUMOylase SENP7-Mediated Epithelial Signaling Triggers Intestinal Inflammation via Expansion of Gamma-Delta T Cells
    Authors: A Suhail, ZA Rizvi, P Mujagond, SA Ali, P Gaur, M Singh, V Ahuja, A Awasthi, CV Srikanth
    Cell Rep, 2019-12-10;29(11):3522-3538.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. TCR-pMHC encounter differentially regulates transcriptomes of(-) tissue-resident CD8 T cells
    Authors: A Yoshikawa, K Bi, DB Keskin, G Zhang, B Reinhold, EL Reinherz
    Eur. J. Immunol., 2017-09-29;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival
    Authors: W Rabacal, SK Pabbisetty, KL Hoek, D Cendron, Y Guo, D Maseda, E Sebzda
    Proc Natl Acad Sci USA, 2016-04-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. ESAT-6-dependent cytosolic pattern recognition drives noncognate tuberculosis control in vivo
    Authors: A Kupz, U Zedler, M Stäber, C Perdomo, A Dorhoi, R Brosch, SH Kaufmann
    J Clin Invest, 2016-04-25;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Maltose-binding protein fusion allows for high level bacterial expression and purification of bioactive mammalian cytokine derivatives.
    Authors: Pennati A, Deng J, Galipeau J
    PLoS ONE, 2014-09-08;9(9):e106724.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Combination of TACI-IgG and anti-IL-15 treats murine lupus by reducing mature and memory B cells.
    Authors: Ma N, Xiao H, Marrero B, Xing C, Wang X, Zheng M, Han G, Chen G, Hou C, Shen B, Li Y, Wang R, Jiang Z
    Cell Immunol, 2014-04-12;289(1):140-4.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. IL-15 complexes induce NK- and T-cell responses independent of type I IFN signaling during rhinovirus infection.
    Authors: Jayaraman A, Jackson D, Message S, Pearson R, Aniscenko J, Caramori G, Mallia P, Papi A, Shamji B, Edwards M, Westwick J, Hansel T, Stanciu L, Johnston S, Bartlett N
    Mucosal Immunol, 2014-01-29;7(5):1151-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  10. Blockade of IL-15 activity inhibits microglial activation through the NFkappaB, p38, and ERK1/2 pathways, reducing cytokine and chemokine release.
    Authors: Gomez-Nicola D, Valle-Argos B, Nieto-Sampedro M
    Glia, 2010-02-01;58(3):264-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  11. IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation.
    Authors: Mortier E, Woo T, Advincula R, Gozalo S, Ma A
    J. Exp. Med., 2008-05-05;205(5):1213-25.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development
  12. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
    Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
    Blood, 2006-11-02;109(5):2234-42.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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