Mouse IL-15 Biotinylated Antibody Summary
Asn49-Ser162
Accession # P48346
Applications
Mouse IL-15 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑15 in Mouse Intestine. IL-15 was detected in perfusion fixed frozen sections of mouse intestine using Mouse IL-15 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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IL‑15 in Mouse Intestine. IL-15 was detected in perfusion fixed frozen sections of mouse intestine using Mouse IL-15 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-15
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1 - 3). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11 - 13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
- Grabstein, K. et al. (1994) Science 264:965.
- Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
- Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
- Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
- Giri, J.G. et al. (1995) EMBO J. 14:3654.
- Giri, J. et al. (1994) EMBO J. 13:2822.
- Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
- Dubois, S. et al. (2002) Immunity 17:537.
- Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
- Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
- Mortier, E. et al. (2004) J. Immunol. 173:1681.
- Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
- Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
- Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Product Datasheets
Citations for Mouse IL-15 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Synergistic Combination of Oncolytic Virotherapy and Immunotherapy for Glioma
Authors: Bingtao Tang, Zong Sheng Guo, David L. Bartlett, David Z. Yan, Claire P. Schane, Diana L. Thomas et al.
Clinical Cancer Research
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The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
Authors: Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S
J. Immunol., 2012-09-19;189(9):4237-46.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
Authors: Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW
J. Immunol., 2009-06-15;182(12):7398-407.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans.
Authors: Rowley J, Monie A, Hung CF, Wu TC
J. Immunol., 2008-12-15;181(12):8237-47.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation.
Authors: Mortier E, Woo T, Advincula R, Gozalo S, Ma A
J. Exp. Med., 2008-05-05;205(5):1213-25.
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA Development -
IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.
Authors: Watkins SK, Egilmez NK, Suttles J, Stout RD
J. Immunol., 2007-02-01;178(3):1357-62.
Species: Mouse
Sample Types: Serum, Whole Cells
Applications: ELISA Development, Flow Cytometry
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Used as a detection antibody in our Mosue Elisa.
