Home / IL-18/IL-1F4 / Mouse IL-18/IL-1F4 Biotinylated Antibody

Mouse IL-18/IL-1F4 Biotinylated Antibody

Catalog #: D048-6
8 Citations Datasheet / COA / SDS
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D048-6
Product Details
Citations (8)
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Mouse IL-18/IL-1F4 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
This antibody reacts with mouse IL-18 on ELISA.
Source
Monoclonal Rat IgG1 Clone # 93-10C
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse IL-18/IL-1F4
Formulation
In 50 μL volume of PBS containing 1% BSA, 10% glycerol and 0.09% sodium azide, pH 7.2.
Label
Biotin

Applications

Recommended Concentration
Sample

Mouse IL-18/IL-1F4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Mouse IL‑18/IL‑1F4 Antibody (Catalog # D047-3)

Standard: Recombinant Mouse IL-18/IL-1F4 Protein (Catalog # B002-5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
 This antibody solution is stable for one year from the date of purchase when stored at 4°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References
  1. McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
  2. Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
  3. Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
  4. Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
  5. Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
  6. Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
  7. Okamura, H., et al. Nature 378, 88-91 (1995)
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

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Citations for Mouse IL-18/IL-1F4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Riboflavin, vitamin B2, attenuates NLRP3, NLRC4, AIM2, and non-canonical inflammasomes by the inhibition of caspase-1 activity
    Authors: H Ahn, GS Lee
    Sci Rep, 2020-11-05;10(1):19091.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  2. Fructose-arginine, a non-saponin molecule of Korean Red Ginseng, attenuates AIM2 inflammasome activation
    Authors: Huijeong Ahn, Byung-Cheol Han, Seung-Ho Lee, Geun-Shik Lee
    Journal of Ginseng Research
  3. Unveiling the Crucial Role of Type IV Secretion System and Motility of Helicobacter pylori in IL-1 beta Production via NLRP3 Inflammasome Activation in Neutrophils
    Authors: Ah-Ra Jang, Min-Jung Kang, Jeong-Ih Shin, Soon-Wook Kwon, Ji-Yeon Park, Jae-Hun Ahn et al.
    Frontiers in Immunology
  4. Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury
    Authors: GM Castillo, A Nishimoto-, CC Jones, KK Kabirov, A Zakharov, AV Lyubimov
    PLoS ONE, 2017-02-16;12(2):e0171703.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  5. NLRP3 Inflammasome: Key Mediator of Neuroinflammation in Murine Japanese Encephalitis
    Authors: Deepak Kumar Kaushik, Malvika Gupta, Kanhaiya Lal Kumawat, Anirban Basu
    PLoS ONE
  6. Induction of IL-33 expression and activity in central nervous system glia.
    Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
    J. Leukoc. Biol., 2008-06-13;84(3):631-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  8. Autophagy in Dendritic Cells and B Cells Is Critical for the Inflammatory State of TLR7-Mediated Autoimmunity
    Authors: Chi G Weindel
    J. Immunol, 2016-12-28;0(0):.

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