Mouse IL-19 Antibody

Catalog #: MAB29151
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29151
MAB29151-SP

All IL-19 Antibodies

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Citations (1)

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Mouse IL-19 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human
IL-19 is observed.

Source

Monoclonal Rat IgG_2B Clone # 350105

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL-19
Leu25-Ala176
Accession # Q8CJ70

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑19 (Catalog # 2915-IL)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

Mouse whole blood cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-19

Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24) (2‑4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). Based on human studies, it is expected to be secreted as a glycosylated monomer, 35‑45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity to unpublished Genbank sequences for rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL-19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

References

Fickenscher, H. et al. (2002) Trends Immunol. 23:89.
Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Zdanov (2004) Curr. Pharm. Des. 10:3873.
Gallagher, G. et al. (2004) Int. Immunopathol. 4:615.
Laio, Y.-C. et al. (2002) J. Immunol. 169:4288.
Gallagher, G. et al. (2000) Genes Immun. 1:442.
Pletnev, S. et al. (2003) Biochemistry 42:12617.
Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
Wolk, K. et al. (2002) J. Immunol. 168:5397.
Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
Laio, S.-C. et al. (2004) J. Immunol. 173:6712.

Long Name

Interleukin 19

Entrez Gene IDs

29949 (Human); 329244 (Mouse)

Alternate Names

IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19

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Citation for Mouse IL-19 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1