Mouse IL-21 Biotinylated Antibody

Catalog #: BAF594
8 Citations Datasheet / COA / SDS

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BAF594

All IL-21 Antibodies

Product Details

Citations (8)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-21 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-21 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL‑15, and recombinant human IL‑21 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
Pro25-Ser146
Accession # Q9ES17

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑21 (Catalog # 594-ML)

Mouse IL-21 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse IL‑21 Antibody (Catalog # AF594)

Standard: Recombinant Mouse IL-21 Protein (Catalog # 594-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma _c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

References

Parrish-Novak, et al. (2000) Nature 408:57.
Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
Asao, H. et al. (2001) J. Immunol. 167:1.

Long Name

Interleukin 21

Entrez Gene IDs

59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)

Alternate Names

CVID11; IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform; Za11

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-21 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Treatment with a Lactococcus lactis that chromosomally express E. coli cfaI mitigates salivary flow loss in a Sjögren's syndrome-like disease
Authors: Akgul, A;Freguia, CF;Maddaloni, M;Hoffman, C;Voigt, A;Nguyen, CQ;Fanger, NA;Fanger, GR;Pascual, DW;
Scientific reports
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Detection
Prostaglandin E2 inhibits Tr1 cell differentiation through suppression of c-Maf
Authors: KM Hooper, W Kong, D Ganea
PLoS ONE, 2017-06-12;12(6):e0179184.
Species: Mouse
Sample Types: Cell Culture Supernates
B-Cell Cross-Presentation of Autologous Antigen Precipitates Diabetes
Authors: Eliana Mariño, Bernice Tan, Lauren Binge, Charles R. Mackay, Shane T. Grey
Diabetes
Interleukin-21 is critically required in autoimmune and allogeneic responses to islet tissue in murine models.
Authors: McGuire HM, Walters S, Vogelzang A
Diabetes, 2011-03-01;60(3):867-75.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Regulation of the IL-21 gene by the NF-kappaB transcription factor c-Rel.
Authors: Chen G, Hardy K, Bunting K
J. Immunol., 2010-07-16;185(4):2350-9.
Species: Mouse
Sample Types: Serum, Whole Cells
Applications: ELISA Development, Flow Cytometry
IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).
Authors: Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW
PLoS ONE, 2010-01-14;5(1):e8720.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Cytokine-secreting follicular T cells shape the antibody repertoire.
Authors: Reinhardt RL, Liang HE, Locksley RM
Nat. Immunol., 2009-03-01;10(4):385-93.
Species: Mouse
Sample Types: Whole Cells
Applications: ELISpot Development
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood, 2008-03-19;112(2):362-73.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development