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Mouse IL-22 DuoSet ELISA

Catalog #: DY582
31 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY582
DY582-05
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
DY009B: DuoSet ELISA Ancillary Reagent Kit 3
Mouse IL-22 ELISA Standard Curve
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Product Details
Procedure
Citations (31)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IL-22 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-22. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 3 (5 plates): (Catalog # DY009) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 3.

Normal Goat Serum: (Catalog # DY005)

 

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY004), or 5% Tween 20 in PBS, 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Normal Goat Serum: (Catalog # DY005)

 

Scientific Data

N/A Mouse IL-22 ELISA Standard Curve View Larger

Mouse IL-22 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-22

IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.

Long Name:
Interleukin 22
Entrez Gene IDs:
50616 (Human); 50929 (Mouse); 500836 (Rat)
Alternate Names:
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18

Assay Procedure

GENERAL ELISA PROTOCOL


Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL of Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent with NGS, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IL-22 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Cell-intrinsic regulation of phagocyte function by interferon lambda during pulmonary viral, bacterial super-infection
    Authors: Antos, D;Parks, OB;Duray, AM;Abraham, N;Michel, JJ;Kupul, S;Westcott, R;Alcorn, JF;
    PLoS pathogens
    Species: Mouse
    Sample Types: BALF
  2. Lipid-encapsulated mRNA encoding an extended serum half-life interleukin-22 ameliorates metabolic disease in mice
    Authors: Canali, S;Fischer, AW;Nguyen, M;Anderson, K;Wu, L;Graham, AR;Hsiao, CJ;Bankar, C;Dussault, N;Ritchie, V;Goodridge, M;Sparrow, T;Pannoni, A;Tse, SW;Woo, V;Klovdahl, K;Iacovelli, J;Huang, E;
    Molecular metabolism
    Species: Mouse
    Sample Types: Serum
  3. IL-10 inhibition during immunization improves vaccine-induced protection against Staphylococcus aureus infection
    Authors: Kelly, AM;McCarthy, KN;Claxton, TJ;Carlile, SR;O'Brien, EC;Vozza, EG;Mills, KH;McLoughlin, RM;
    JCI insight
    Species: Mouse
    Sample Types: Cell Culture Supernates, Tissue Homogenates
  4. Mucosal TLR5 activation controls healthspan and longevity
    Authors: Lim, JS;Jeon, EJ;Go, HS;Kim, HJ;Kim, KY;Nguyen, TQT;Lee, DY;Kim, KS;Pietrocola, F;Hong, SH;Lee, SE;Kim, KS;Park, TS;Choi, DH;Jeong, YJ;Park, JH;Kim, HS;Min, JJ;Kim, YS;Park, JT;Cho, JH;Lee, GW;Lee, JH;Choy, HE;Park, SC;Lee, CH;Rhee, JH;Serrano, M;Cho, KA;
    Nature communications
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Role of the IL-33/ST2 Activation Pathway in the Development of the Hepatic Fibrosis Induced by Schistosoma mansoni Granulomas in Mice
    Authors: Maggi, L;Camelo, GMA;Rocha, IC;Pereira Alves, W;Moreira, JMP;Almeida Pereira, T;Tafuri, WL;Rabelo, ÉML;Correa, A;Ecco, R;Negrão-Corrêa, DA;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Serum, Tissue Homogenates
  6. ERdj5 protects goblet cells from endoplasmic reticulum stress-mediated apoptosis under inflammatory conditions
    Authors: H Jeong, EH Hong, JH Ahn, J Cho, JH Jeong, CW Kim, BI Yoon, JH Koo, YY Park, YM Yang, T Iwawaki, BA Vallance, SY Chang, HJ Ko
    Experimental & Molecular Medicine, 2023-02-09;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Antimicrobial overproduction sustains intestinal inflammation by inhibiting Enterococcus colonization
    Authors: KK Jang, T Heaney, M London, Y Ding, F Yeung, D Ercelen, YH Chen, J Axelrad, S Gurunathan, A Marijke Ke, ME Griffin, HC Hang, K Cadwell
    bioRxiv : the preprint server for biology, 2023-02-01;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. IL-22 regulates inflammatory responses to agricultural dust-induced airway inflammation
    Authors: A Ulu, S Sveiven, A Bilg, JV Velazquez, M Diaz, M Mukherjee, AG Yuil-Valde, S Kota, A Burr, A Najera, TM Nordgren
    Toxicology and Applied Pharmacology, 2022-05-05;0(0):116044.
    Species: Mouse
    Sample Types: BALF
  9. ADP-ribosylating adjuvant reveals plasticity in cDC1 cells that drive mucosal Th17 cell development and protection against influenza virus infection
    Authors: M Arabpour, C Lebrero-Fe, K Schön, A Strömberg, V Börjesson, K Lahl, M Ballegeer, X Saelens, D Angeletti, W Agace, N Lycke
    Mucosal Immunology, 2022-04-13;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Effects of repeated infections with non-typeable Haemophilus influenzae on lung in vitamin D deficient and smoking mice
    Authors: J Serré, AT Tanjeko, C Mathyssen, T Heigl, A Sacreas, DP Cook, E Verbeken, K Maes, J Verhaegen, C Pilette, J Vanoirbeek, C Gysemans, C Mathieu, B Vanaudenae, W Janssens, G Gayan-Rami
    Respiratory Research, 2022-03-02;23(1):40.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Geographic differences in gut microbiota composition impact susceptibility to enteric infection
    Authors: AM Porras, Q Shi, H Zhou, R Callahan, G Montenegro, N Solomons, IL Brito
    Cell Reports, 2021-07-27;36(4):109457.
    Species: Mouse
    Sample Types: Tissue Supernates
  12. Aryl hydrocarbon receptor (Ahr)-dependent Il-22 expression by type 3 innate lymphoid cells control of acute joint inflammation
    Authors: R Nehmar, L Fauconnier, J Alves-Filh, D Togbe, A DeCauwer, S Bahram, M Le Bert, B Ryffel, P Georgel
    Journal of Cellular and Molecular Medicine, 2021-03-18;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. GM-CSF Calibrates Macrophage Defense and Wound Healing Programs during Intestinal Infection and Inflammation
    Authors: T Castro-Dop, A Fleming, TW Dennison, JR Ferdinand, K Harcourt, BJ Stewart, Z Cader, ZK Tuong, C Jing, LSC Lok, RJ Mathews, A Portet, A Kaser, S Clare, MR Clatworthy
    Cell Rep, 2020-07-07;32(1):107857.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Lactobacillus reuteri 5454 and Bifidobacterium animalis ssp. lactis 5764 improve colitis while differentially impacting dendritic cells maturation and antimicrobial responses
    Authors: J Hrdý, J Alard, A Couturier-, O Boulard, D Boutillier, M Delacre, C Lapadatesc, A Cesaro, P Blanc, B Pot, B Ryffel, M Chamaillar, C Grangette
    Sci Rep, 2020-03-24;10(1):5345.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. PAD4-dependent NETs generation are indispensable for intestinal clearance of Citrobacter rodentium
    Authors: P Saha, BS Yeoh, X Xiao, RM Golonka, V Singh, Y Wang, M Vijay-Kuma
    Mucosal Immunol, 2019-02-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and antimicrobial responses
    Authors: CN Berger, VF Crepin, TI Roumelioti, JC Wright, N Serafini, M Pevsner-Fi, L Yu, E Elinav, JP Di Santo, JS Choudhary, G Frankel
    PLoS Pathog., 2018-10-26;14(10):e1007406.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Integration of T Cell Receptor, Notch and Cytokine Signals Programs in Mouse ?? T Cell Effector Differentiation
    Authors: P Zarin, TSH In, ELY Chen, J Singh, GW Wong, M Mohtashami, DL Wiest, MK Anderson, JC Zúñiga-Pfl
    Immunol. Cell Biol., 2018-06-28;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Th17 cells are refractory to senescence and retain robust antitumor activity after long-term ex vivo expansion
    Authors: JS Bowers, MH Nelson, K Majchrzak, SR Bailey, B Rohrer, AD Kaiser, C Atkinson, L Gattinoni, CM Paulos
    JCI Insight, 2017-03-09;2(5):e90772.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. T-bet overexpression regulates AHR-mediated Th-17 differentiation through an IFN?-independent pathway
    Authors: Masahiro Yokosawa
    Clin. Exp. Immunol, 2017-01-31;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Interleukin 7 immunotherapy improves host immunity and survival in a two-hit model of Pseudomonas aeruginosa pneumonia
    J Leukoc Biol, 2016-09-14;0(0):.
    Species: Mouse
    Sample Types: Plasma
  21. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.
    Authors: Brown A, Murphy A, Lalor S, Leech J, O'Keeffe K, Mac Aogain M, O'Halloran D, Lacey K, Tavakol M, Hearnden C, Fitzgerald-Hughes D, Humphreys H, Fennell J, van Wamel W, Foster T, Geoghegan J, Lavelle E, Rogers T, McLoughlin R
    PLoS Pathog, 2015-11-05;11(11):e1005226.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Cholera toxin adjuvant promotes a balanced Th1/Th2/Th17 response independently of IL-12 and IL-17 by acting on Gsalpha in CD11b(+) DCs.
    Authors: Mattsson J, Schon K, Ekman L, Fahlen-Yrlid L, Yrlid U, Lycke N
    Mucosal Immunol, 2014-11-26;8(4):815-27.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. The aryl hydrocarbon receptor: differential contribution to T helper 17 and T cytotoxic 17 cell development.
    Authors: Hayes M, Ovcinnikovs V, Smith A, Kimber I, Dearman R
    PLoS ONE, 2014-09-09;9(9):e106955.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Lymphotoxin beta receptor signaling limits mucosal damage through driving IL-23 production by epithelial cells.
    Authors: Macho-Fernandez E, Koroleva E, Spencer C, Tighe M, Torrado E, Cooper A, Fu Y, Tumanov A
    Mucosal Immunol, 2014-09-03;8(2):403-13.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. Unique macrophages different from M1/M2 macrophages inhibit T cell mitogenesis while upregulating Th17 polarization.
    Authors: Tatano Y, Shimizu T, Tomioka H
    Sci Rep, 2014-02-20;4(0):4146.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.
    Authors: van Voorhis, Michael, Knopp, Samantha, Julliard, Walker, Fechner, John H, Zhang, Xiaoji, Schauer, James J, Mezrich, Joshua D
    PLoS ONE, 2013-12-11;8(12):e82545.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Influence of hypoxia-inducible factor 1-alpha on dendritic cell differentiation and migration.
    Authors: Kohler T, Reizis B, Johnson RS, Weighardt H, Forster I
    Eur. J. Immunol., 2012-05-01;42(5):1226-36.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Interleukin-23-mediated inflammation in Pseudomonas aeruginosa pulmonary infection.
    Authors: Dubin PJ, Martz A, Eisenstatt JR, Fox MD, Logar A, Kolls JK
    Infect. Immun., 2011-10-24;80(1):398-409.
    Species: Mouse
    Sample Types: BALF
  29. High expression of IL-22 suppresses antigen-induced immune responses and eosinophilic airway inflammation via an IL-10-associated mechanism.
    Authors: Nakagome K, Imamura M, Kawahata K
    J. Immunol., 2011-10-12;187(10):5077-89.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
    Authors: El-Behi M, Ciric B, Dai H
    Nat. Immunol., 2011-04-24;12(6):568-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. IL-1RL2 and its ligands contribute to the cytokine network in psoriasis.
    Authors: Blumberg H, Dinh H, Dean C, Trueblood ES, Bailey K, Shows D, Bhagavathula N, Aslam MN, Varani J, Towne JE, Sims JE
    J. Immunol., 2010-09-10;185(7):4354-62.
    Species: Mouse
    Sample Types: Tissue Homogenates

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