Mouse IL-22 R alpha 1 Antibody

Catalog #: AF4294 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4294
AF4294-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-22 R alpha 1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑22 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human IL‑22 R is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-22 R alpha 1
Thr18-Ala228
Accession # Q80XZ4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑22 R alpha 1 (Catalog # 4294-MR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22 R alpha 1

The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic domain (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20%‑26% aa sequence identity with the ECDs of other class II receptors IL‑10 R, IL‑20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R beta is an IL‑20 or IL‑24 responsive receptor (5, 6). In both cases, IL‑22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R beta or IL-20 R beta serves to stabilize the complex (3, 6‑9). IL‑22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R beta or IL-20 R beta is required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL‑22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11‑14). IL‑22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL‑22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).

References

Tachiiri, A. et al. (2003) Genes Immun. 4:153.
Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
Boniface, K. et al. (2005) J. Immunol. 174:3695.
Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099
Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
Wolk, K. et al. (2004) Immunity 21:241.
Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

Long Name

Interleukin 22 Receptor

Entrez Gene IDs

58985 (Human); 230828 (Mouse)

Alternate Names

CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11