Mouse IL-27 p28 Alexa Fluor® 405-conjugated Antibody

Catalog #: IC1834V Datasheet / COA / SDS

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IC1834V-100UG

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Mouse IL-27 p28 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-27 in Western blots. This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit.

Source

Monoclonal Rat IgG2b Clone # 234205

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse IL‑27
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25-1 µg/10⁶ cells

RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-27 p28/IL-30

Interleukin 27 (IL-27) is a novel heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI-3 (Epstein‑Barr virus‑induced gene 3). IL-27 binds TCCR/WSX-1 on naïve CD4⁺ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL‑12‑mediated Th1 cell development.

Long Name

Interleukin 27 p28

Entrez Gene IDs

246778 (Human); 246779 (Mouse)

Alternate Names

IL27 p28; IL-27 p28; IL27;IL-27;IL27A;IL-27A;IL27p28;IL-27p28;IL30;p28; IL-30

Product Datasheets

Product Datasheet

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.