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Mouse IL-27 p28/IL-30 Antibody

Catalog #: AF1834
18 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1834
AF1834-SP
IL‑27 p28/IL‑30 in Mouse Thymus.
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Citations (18)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-27 p28/IL-30 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-27 p28/IL-30 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-27 p28/IL-30
Phe29-Ser234
Accession # Q8K3I6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑27 p28/IL-30
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑27 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Bender, H. et al. (2009) Hepatology 50:585. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑27.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IL-27 p28/IL-30 antibody in Mouse Thymus by Immunohistochemistry (IHC-P). View Larger

IL‑27 p28/IL‑30 in Mouse Thymus. IL-27 p28/IL-30 was detected in perfusion fixed paraffin-embedded sections of mouse thymus using 15 µg/mL Mouse IL-27 p28/ IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization IL‑27 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑27 p28/IL‑30 Antibody. View Larger

IL‑27 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑27 p28/IL‑30 Antibody. Recombinant Mouse IL-27 (Catalog # 2799-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IL-27 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-27 p28/IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834). The ND50 is typically 1-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27 p28/IL-30

 IL-27 p28 subunit (also known as IL-30 and Interleukin-27 subunit alpha) is a 26 kDa glycoprotein. IL-27 is a heterodimeric cytokine consisting of the IL-27 p28 subunit that is an IL-12 p35 related protein and EBI3, which is related to IL-12 p40. The biological effects of IL-27 are mediated by the heteromeric receptor complex gp130 and TCCR/WSX-1.

Long Name
Interleukin 27 p28
Entrez Gene IDs
246778 (Human); 246779 (Mouse)
Alternate Names
IL27 p28; IL-27 p28; IL27;IL-27;IL27A;IL-27A;IL27p28;IL-27p28;IL30;p28; IL-30

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Citations for Mouse IL-27 p28/IL-30 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis
    Authors: Mengmeng Zhao, Zihui Zheng, Pingan Zhang, Yao Xu, Jishou Zhang, Shanshan Peng et al.
    iScience
  2. CRISPR/Cas9-mediated deletion of Interleukin-30 suppresses IGF1 and CXCL5 and boosts SOCS3 reducing prostate cancer growth and mortality
    Authors: C Sorrentino, L D'Antonio, SL Ciummo, C Fieni, L Landuzzi, F Ruzzi, S Vespa, P Lanuti, LV Lotti, PL Lollini, E Di Carlo
    Journal of hematology & oncology, 2022-10-13;15(1):145.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. EBV-induced gene 3 augments IL-23R&alpha protein expression through a chaperone calnexin
    Authors: I Mizoguchi, M Ohashi, H Hasegawa, Y Chiba, N Orii, S Inoue, C Kawana, M Xu, K Sudo, K Fujita, M Kuroda, SI Hashimoto, K Matsushima, T Yoshimoto
    J. Clin. Invest., 2020-11-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Interleukin-33 activates regulatory T cells to suppress innate &gamma&delta T cell responses in the lung
    Authors: LD Faustino, JW Griffith, RA Rahimi, K Nepal, DL Hamilos, JL Cho, BD Medoff, JJ Moon, DAA Vignali, AD Luster
    Nat Immunol, 2020-09-28;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Glycosaminoglycans bind human IL-27 and regulate its activity
    Authors: MC Cavé, S Maillard, K Hildenbran, C Mamelonet, MJ Feige, O Devergne
    Eur. J. Immunol., 2020-07-02;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Capture
  6. Interleukin-27 Early Impacts Leishmania infantum Infection in Mice and Correlates with Active Visceral Disease in Humans
    Authors: Anabela Cordeiro-d
    Front Immunol, 2016-11-04;7(0):478.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes
    Sci Rep, 2016-07-29;6(0):30448.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor
    Nat Med, 2016-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Trypanosoma cruzi Infection Imparts a Regulatory Program in Dendritic Cells and T Cells via Galectin-1-Dependent Mechanisms.
    Authors: Poncini C, Ilarregui J, Batalla E, Engels S, Cerliani J, Cucher M, van Kooyk Y, Gonzalez-Cappa S, Rabinovich G
    J Immunol, 2015-08-31;195(7):3311-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Attenuation of intestinal inflammation in interleukin-10-deficient mice infected with Citrobacter rodentium.
    Authors: Dann, Sara M, Le, Christin, Choudhury, Barun K, Liu, Houpu, Saldarriaga, Omar, Hanson, Elaine M, Cong, Yingzi, Eckmann, Lars
    Infect Immun, 2014-02-24;82(5):1949-58.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. Control of T cell fates and immune tolerance by p38 alpha signaling in mucosal CD103+ dendritic cells1
    Authors: Gonghua Huang, Yanyan Wang, Hongbo Chi
    The Journal of Immunology
  12. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
    Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
    J Immunol, 2012-11-14;189(12):5590-601.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis.
    Authors: Owens BM, Beattie L, Moore JW, Brown N, Mann JL, Dalton JE, Maroof A, Kaye PM
    PLoS Pathog., 2012-07-26;8(7):e1002827.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  14. TLR2-mediated production of IL-27 and chemokines by respiratory epithelial cells promotes bleomycin-induced pulmonary fibrosis in mice.
    Authors: Kim HS, Go H, Akira S, Chung DH
    J. Immunol., 2011-09-19;187(8):4007-17.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10.
    Authors: Ilarregui JM, Croci DO, Bianco GA, Toscano MA, Salatino M, Vermeulen ME, Geffner JR, Rabinovich GA
    Nat. Immunol., 2009-08-09;10(9):981-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin.
    Authors: Shinohara ML, Kim JH, Garcia VA, Cantor H
    Immunity, 2008-07-18;29(1):68-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  17. The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice.
    Authors: Guo B, Chang EY, Cheng G
    J. Clin. Invest., 2008-05-01;118(5):1680-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Interleukin-23 and interleukin-27 exert quite different antitumor and vaccine effects on poorly immunogenic melanoma.
    Authors: Oniki S, Nagai H, Horikawa T, Furukawa J, Belladonna ML, Yoshimoto T, Hara I, Nishigori C
    Cancer Res., 2006-06-15;66(12):6395-404.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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