Mouse IL-36 beta /IL-1F8 Antibody

Catalog #: AF2298
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2298
AF2298-SP

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-36 beta /IL-1F8 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑36 beta /IL‑1F8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) IL-1F8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IL‑36 beta /IL‑1F8
Met1-Lys183
Accession # Q9D6Z6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑36 beta /IL‑1F8 (Catalog # 2298-ML)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.8-3.2 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of Recombinant Mouse IL-1 Rrp2/IL-1 R6 Fc Chimera (Catalog # 2354-RP) to immobilized Recombinant Mouse IL‑36 beta /IL‑1F8 (Catalog # 2298-ML) coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 beta/IL-1F8

Mouse interleukin-36 beta [IL-36 beta ; also named IL-1F8, interleukin 1 family member 8, FIL-1 eta (eta) and IL-1H2] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL‑1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -stranded beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Although two alternatively spliced transcript variants for human IL-1F8 have been described, to date, only one mouse IL-1F8 isoform is known (3). Mouse IL-1F8 is synthesized as a 183 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1, 2). It is likely that mouse IL-1F8, similarly to its human homologue, is actively secreted (1). Mouse IL-1F8 shares 61% and 74% aa identity with human IL-1F8 isoform 2 and rat IL-1F8, respectively. Within the IL-1 family, IL-1F8 shares 27% sequence with 33%, 32%, 35%, 39% and 28% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F9 and F10, respectively. Cells reported to express IL-1F8 include resting and activated monocytes and B cells (1, 4). The receptor for IL-1F8 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-1F8, along with IL-1F6 and IL-1F9, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1Rrp2 dependent manner.

References

Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
Dunn, E. et al. (2001) Trends Immunol. 22:533.
Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 36 beta/Interleukin 1 Family 8

Entrez Gene IDs

27177 (Human); 69677 (Mouse)

Alternate Names

family of interleukin 1-eta; FIL1 eta; FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); IL-1 eta; IL1-ETA; IL1F8 (Canonical product IL-1F8a); IL-1F8 (FIL1-eta); IL1F8; IL-1F8; IL1H2; IL-1H2; IL36 beta; IL-36 beta; IL36B; interleukin 1 family, member 8 (eta); interleukin 1, eta; Interleukin 36, Beta; Interleukin-1 eta; interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 Beta

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse IL-36 beta /IL-1F8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Epithelial cells release IL-36 alpha in extracellular vesicles following mechanical damage
Authors: Drake Winslow Williams, Reuben H. Kim
Biochemical and Biophysical Research Communications
MES SV40 Cells Are Sensitive to Lipopolysaccharide, Peptidoglycan, and Poly I:C Expressing IL-36 Cytokines
Authors: CG Pelcastre-, EA Vazquez-Sa, JM Murrieta-C, S Rodríguez-, JC Cancino-Di, ME Cancino-Di
International Journal of Molecular Sciences, 2022-10-07;23(19):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Unprocessed Interleukin-36alpha Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1.
Authors: Milora K, Fu H, Dubaz O, Jensen L
J Invest Dermatol, 2015-07-23;135(12):2992-3000.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes.
Authors: Magne D, Palmer G, Barton JL, Mezin F, Talabot-Ayer D, Bas S, Duffy T, Noger M, Guerne PA, Nicklin MJ, Gabay C
Arthritis Res. Ther., 2006-04-28;8(3):R80.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development