Mouse IL-6 Antibody

Catalog #: AB-406-NA
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AB-406-NA

Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody.

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Citations (8)

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Mouse IL-6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant rat IL‑6 is observed and less than 5% cross-reactivity with recombinant human IL‑6, recombinant feline IL‑6, recombinant bovine IL‑6, and recombinant porcine IL‑6 is observed.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse IL-6
Phe25-Thr211
Accession # P08505

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑6 (Catalog # 406-ML)

Neutralization

Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line [Nordan, R.P. and M. Potter (1986) Science 233:566]. The Neutralization Dose (ND₅₀) is typically 0.05-0.15 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody. Recombinant Mouse IL-6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Polyclonal Antibody (Catalog # AB-406-NA). The ND₅₀ is typically 0.05-0.15 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature mouse IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Mouse IL-6 shares 39% and 85% aa sequence identity with human and rat IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name

Interleukin 6

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Alternate Names

B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

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Citations for Mouse IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Anti-diabetic effects of GLP1 analogs are mediated by thermogenic interleukin-6 signaling in adipocytes
Authors: Absalon D. Gutierrez, Zhanguo Gao, Vala Hamidi, Liang Zhu, Karla Bermudez Saint Andre, Kayla Riggs et al.
Cell Reports Medicine
Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration
Authors: X Liu, E Zu, X Chang, X Ma, Z Wang, X Song, X Li, Q Yu, KI Kamei, T Hayashi, K Mizuno, S Hattori, H Fujisaki, T Ikejima, DO Wang
Disease Models & Mechanisms, 2021-12-24;14(12):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Tyrosyl phosphorylation of PZR promotes hypertrophic cardiomyopathy in PTPN11-associated Noonan syndrome with multiple lentigines
Authors: JS Yi, SK Perla, LE Enyenihi, AM Bennett
JCI Insight, 2020-08-06;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing
Authors: S Mahmoudi, E Mancini, L Xu, A Moore, F Jahanbani, K Hebestreit, R Srinivasan, X Li, K Devarajan, L Prélot, CE Ang, Y Shibuya, BA Benayoun, ALS Chang, M Wernig, J Wysocka, MT Longaker, MP Snyder, A Brunet
Nature, 2019-10-23;574(7779):553-558.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture
Exercise Protects Against Olanzapine-Induced Hyperglycemia in Male C57BL/6J Mice
Authors: Laura N. Castellani, Willem T. Peppler, Paula M. Miotto, Natasha Bush, David C. Wright
Scientific Reports
TGF-beta -induced IL-6 prevents development of acute lung injury in influenza A virus-infected F508del CFTR-heterozygous mice
Authors: Parker S. Woods, Mia F. Tazi, Nicholas M. Chesarino, Amal O. Amer, Ian C. Davis
American Journal of Physiology-Lung Cellular and Molecular Physiology
Retinol-Binding Protein 4 Inhibits Insulin Signaling in Adipocytes by Inducing Proinflammatory Cytokines in Macrophages through a c-Jun N-Terminal Kinase- and Toll-Like Receptor 4-Dependent and Retinol-Independent Mechanism
Authors: Julie Norseen, Tetsuya Hosooka, Ann Hammarstedt, Mark M. Yore, Shashi Kant, Pratik Aryal et al.
Molecular and Cellular Biology
Maladaptive role of IL-6 in ischemic acute renal failure.
Authors: Kielar ML, John R, Bennett M
J. Am. Soc. Nephrol., 2005-09-28;16(11):3315-25.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization