Mouse IL-6R alpha Antibody Summary
Leu20-Glu357
Accession # P22272
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL-6R alpha in Mouse Splenocytes. IL-6R alpha was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse IL-6R alpha Monoclonal Antibody (Catalog # MAB18301) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6R alpha
Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL‑6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL‑6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL‑6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).
- Yamasaki, K. et al. (1988) Science 241:825.
- Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
- Hibi, M. et al. (1990) Cell 63:1149.
- Saito, M. et al. (1992) J. Immunol. 148:4066.
- Novick, D. et al. (1989) J. Exp. Med. 170:1409.
- Jones, S.A. et al. (2001) FASEB J. 15:43.
- Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.
Product Datasheets
Citations for Mouse IL-6R alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Sertoli cell-initiated testicular innate immune response through toll-like receptor-3 activation is negatively regulated by Tyro3, Axl, and mer receptors.
Authors: Sun B, Qi N, Shang T
Endocrinology, 2010-04-02;151(6):2886-97.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
Functional interleukin-6 receptor-alpha is located in tanycytes at the base of the third ventricle
Authors: F. Anesten, C. Santos, E. Gidestrand, E. Schéle, V. Pálsdóttir, T. Swedung-Wettervik et al.
Journal of Neuroendocrinology
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